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    Displacement polynucleotide assay method and polynucleotide complex reagent therefor
    1.
    发明公开
    Displacement polynucleotide assay method and polynucleotide complex reagent therefor 失效
    置换多核苷酸分析方法及其多核苷酸复合物试剂

    公开(公告)号：EP0167238A1

    公开(公告)日：1986-01-08

    申请号：EP85303175.5

    申请日：1985-05-03

    申请人： ALLIED CORPORATION ,  GENETICS INSTITUTE, INC.

    发明人： Collins, Mary ,  Fritsch, Edward Francis ,  Williams, Jon Ira ,  Brewen, Joseph Grant ,  Diamond, Steven Elliot ,  Ellwood, Marian Sue

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/6876 ,  Y10S435/803 ,  Y10S435/81

    摘要： A diagnostic reagent is disclosed containing a complex of a probe polynucleotide (P) bound via purine/pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) contains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the target nucleotide sequence (G) causes binding, initially between G and a single-stranded portion (IBR) of the target binding region (TBR). Thereafter the labeled polynucleotide (L) is displaced from the complex by branch migration of (G) into the (P)/(L) binding region. Determination of displaced labeled polynucleotide (L) gives a value which is a function of the presence and concentration of target nucleotide sequence (G) in the sample.

    摘要翻译： 公开了一种诊断试剂，其含有通过与标记的多核苷酸（L）氢键结合的嘌呤/嘧啶结合的探针多核苷酸（P）的复合物。 探针（P）包含能够结合生物样品的靶核苷酸序列（G）的靶结合区域（TBR）。 公开了一种方法，其中与包含靶核苷酸序列（G）的样品的接触初始在G与靶结合区域（TBR）的单链部分（IBR）之间引起结合。 此后，标记的多核苷酸（L）通过（G）的分支迁移到（P）/（L）结合区域从复合体中置换。 置换标记多核苷酸（L）的测定给出的值是样品中靶核苷酸序列（G）的存在和浓度的函数。

    Displacement polynucleotide assay employing recombination protein and diagnostic kit
    3.
    发明公开
    Displacement polynucleotide assay employing recombination protein and diagnostic kit 失效
    Polynukleotidanalyse durch Verschiebung unter Verwendung eines Rekombinationsproteins und Reagenzsatz。

    公开(公告)号：EP0164876A1

    公开(公告)日：1985-12-18

    申请号：EP85303176.3

    申请日：1985-05-03

    申请人： ALLIED CORPORATION ,  GENETICS INSTITUTE, INC.

    发明人： Collins, Mary ,  Fritsch, Edward Francis ,  Williams, Jon Ira ,  Brewen, Joseph Grant ,  Diamond, Steven Elliot ,  Ellwood, Marian Sue

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6876

    摘要： A diagnostic reagent is disclosed containing a complex of a probe polynucleotide (P) bound via purine/pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) contains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the target nucleotide sequence (G) in the presence of a recombination protein and its cofactors causes binding, initially between G and a single-stranded portion (IBR) of the target binding region (TBR). Thereafter the labeled polynucleotide (L) is displaced from the complex by branch migration of (G) into the (P)/(L) binding region. Determination of displaced labeled polynucleotide (L) gives a value which is a function of the presence and concentration of target nucleotide sequence (G) in the sample. The presence of the recombination protein (such as the rec A protein from the enteric bacterium E. coli) enhances the rate of appearance of displaced labeled polynucleotide, especially when the reaction is conducted at or near physiological temperatures

    摘要翻译： 公开了一种诊断试剂，其含有通过嘌呤/嘧啶氢键与标记的多核苷酸（L）结合的探针多核苷酸（P）的复合物。 探针（P）含有能够结合生物样品的靶核苷酸序列（G）的靶结合区（TBR）。 公开了一种方法，其中在存在重组蛋白及其辅因子的情况下与含有靶核苷酸序列（G）的样品的接触引起最初在G与目标结合区的单链部分（IBR）之间的结合（TBR ）。 此后，通过（G）的分支迁移进入（P）/（L）结合区域，标记的多核苷酸（L）从配合物中移位。 置换的标记多核苷酸（L）的测定给出了样品中靶核苷酸序列（G）的存在和浓度的函数值。 重组蛋白（例如来自肠杆菌大肠杆菌的rec A蛋白）的存在增加了置换的标记多核苷酸的出现速率，特别是当反应在生理温度或接近生理温度下进行时