摘要:
This invention relates to a γ-cyclodextrin glucanotransferase having novel properties, to a process for the production of the γ-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the γ-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of γ-cyclodextrin as a main product, and to a method for increasing γ-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which γ-cyclodextrin and β-cyclodextrin are formed from starch by the action of γ-cyclodextrin glucanotransferase, thereby effecting repressed formation of β-cyclodextrin and enhanced formation of γ-cyclodextrin. Development of the process of this invention has rendered possible low cost production of γ-cyclodextrin and therefore expands practical use of γ-cyclodextrin to the field of food and the like.
摘要:
This invention relates to a γ-cyclodextrin glucanotransferase having novel properties, to a process for the production of the γ-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the γ-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of γ-cyclodextrin as a main product, and to a method for increasing γ-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which γ-cyclodextrin and β-cyclodextrin are formed from starch by the action of γ-cyclodextrin glucanotransferase, thereby effecting repressed formation of β-cyclodextrin and enhanced formation of γ-cyclodextrin. Development of the process of this invention has rendered possible low cost production of γ-cyclodextrin and therefore expands practical use of γ-cyclodextrin to the field of food and the like.