Novel cyclodextrin glucanotransferase, process for producing the same and process for producing cyclodextrin using the same
    1.
    发明公开
    Novel cyclodextrin glucanotransferase, process for producing the same and process for producing cyclodextrin using the same 失效
    新颖环糊精葡聚糖,它们用于通过这个手段制备Cyclodexrin制备和处理方法。

    公开(公告)号:EP0614971A3

    公开(公告)日:1994-11-02

    申请号:EP94101978.8

    申请日:1994-02-09

    IPC分类号: C12N9/10 C12P19/18 C12N1/20

    摘要: This invention relates to a γ-cyclodextrin glucanotransferase having novel properties, to a process for the production of the γ-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the γ-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of γ-cyclodextrin as a main product, and to a method for increasing γ-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which γ-cyclodextrin and β-cyclodextrin are formed from starch by the action of γ-cyclodextrin glucanotransferase, thereby effecting repressed formation of β-cyclodextrin and enhanced formation of γ-cyclodextrin. Development of the process of this invention has rendered possible low cost production of γ-cyclodextrin and therefore expands practical use of γ-cyclodextrin to the field of food and the like.

    Novel cyclodextrin glucanotransferase, process for producing the same and process for producing cyclodextrin using the same
    2.
    发明公开
    Novel cyclodextrin glucanotransferase, process for producing the same and process for producing cyclodextrin using the same 失效
    新颖环糊精葡聚糖,它们用于通过这个手段制备Cyclodexrin制备和处理方法。

    公开(公告)号:EP0614971A2

    公开(公告)日:1994-09-14

    申请号:EP94101978.8

    申请日:1994-02-09

    IPC分类号: C12N9/10 C12P19/18 C12N1/20

    摘要: This invention relates to a γ-cyclodextrin glucanotransferase having novel properties, to a process for the production of the γ-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the γ-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of γ-cyclodextrin as a main product, and to a method for increasing γ-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which γ-cyclodextrin and β-cyclodextrin are formed from starch by the action of γ-cyclodextrin glucanotransferase, thereby effecting repressed formation of β-cyclodextrin and enhanced formation of γ-cyclodextrin. Development of the process of this invention has rendered possible low cost production of γ-cyclodextrin and therefore expands practical use of γ-cyclodextrin to the field of food and the like.

    摘要翻译: 本发明涉及一种γ-环糊精葡聚糖转移酶具有新特性,一种方法,用于生产γ-环糊精葡聚糖转移酶,其包括培养属于能够产生的环糊精葡聚糖转移酶,从而使应变以产生伽马的短杆菌属的菌株 - 环糊精葡聚糖在培养基中,并随后收集酶,一种用于生产的环糊精包括使环糊精葡聚糖转移酶与在溶液中溶解,由此实现形成γ-环糊精的作为主要产物底物反应,并 为增加γ-环糊精产量不伴随总环糊精,该方法包括将乙醇于其中γ-环糊精和β-环糊精的反应溶液通过γ-环糊精葡聚糖转移酶的作用由淀粉形成的产率增加,从而实现repres的方法 β-环糊精的SED的形成和增强γ-环糊精的形成。 本发明的方法的发展已经成为可能低成本生产γ-环糊精,因此扩展到食品领域等实际使用γ-环糊精的。