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公开(公告)号：EP0115284A3
公开(公告)日：1988-01-20
申请号：EP84100299
申请日：1984-01-12
申请人： AMF INCORPORATED
CPC分类号： C12N5/0018 , C12N2500/84
摘要： @ A growth factor containing serum derived from natural bovine serum by precipitating a first solids fraction from natural bovine serum by adding ammonium sulfate to about 25% saturation to give a first solids fraction and a first supernatant; separating said first solids fraction from said first supernatant; precipitating a second solids fractions from said first supernatant by adding ammonium sulfate to about 40% saturation to give a second solids fraction and a second supernatant; separating said second solids fraction from said second supernatant; combining said first solids fraction and said second supernatant; and desalinating said first solids fraction and said second supernatant, either before or after said combining.
摘要翻译： 含有来自天然牛血清的血清的生长因子通过将天然牛血清中的第一固体部分通过加入硫酸铵至约25％的饱和度而得到第一固体部分和第一上清; 将所述第一固体部分与所述第一上清液分离; 通过加入硫酸铵至约40％饱和度从所述第一上清液沉淀第二固体部分，得到第二固体部分和第二上清液; 将所述第二固体部分与所述第二上清液分离; 组合所述第一固体部分和所述第二上清液; 并在所述组合之前或之后脱盐所述第一固体部分和所述第二上清液。
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公开(公告)号：EP0115284A2
公开(公告)日：1984-08-08
申请号：EP84100299.1
申请日：1984-01-12
申请人： AMF INCORPORATED
CPC分类号： C12N5/0018 , C12N2500/84
摘要： @ A growth factor containing serum derived from natural bovine serum by precipitating a first solids fraction from natural bovine serum by adding ammonium sulfate to about 25% saturation to give a first solids fraction and a first supernatant; separating said first solids fraction from said first supernatant; precipitating a second solids fractions from said first supernatant by adding ammonium sulfate to about 40% saturation to give a second solids fraction and a second supernatant; separating said second solids fraction from said second supernatant; combining said first solids fraction and said second supernatant; and desalinating said first solids fraction and said second supernatant, either before or after said combining.
摘要翻译： 含有来自天然牛血清的血清的生长因子通过将天然牛血清中的第一固体部分通过加入硫酸铵至约25％的饱和度而得到第一固体部分和第一上清; 将所述第一固体部分与所述第一上清液分离; 通过加入硫酸铵至约40％饱和度从所述第一上清液沉淀第二固体部分，得到第二固体部分和第二上清液; 将所述第二固体部分与所述第二上清液分离; 组合所述第一固体部分和所述第二上清液; 并在所述组合之前或之后脱盐所述第一固体部分和所述第二上清液。
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