公开(公告)号：EP3531125A1

公开(公告)日：2019-08-28

申请号：EP18305185.3

申请日：2018-02-22

申请人： ASSISTANCE PUBLIQUE, HOPITAUX DE PARIS ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM) ,  UNIVERSITE PARIS DESCARTES ,  Ecole Nationale Superieure de Chimie de Paris

发明人： SALMON, Hugo ,  GAHOUAL, Rabah ,  MIGNET, Nathalie ,  HOUZE, Pascal

IPC分类号： G01N30/00

摘要： The present invention relates to a asymmetric flow field-flow fractionation device (1) configured to separate a samle (8) of particles (12) dispersed in a liquid mobile phase (11), the device including a fractionation microchannel (2) comprising a sample inlet, a sample outlet, an auxiliary microchannel (3) comprising an auxiliary outlet, a semipermeable membrane (10) separating the fractionation microchannel (2) and the auxiliary microchannel (2), said membrane (10) being permeable to liquid and being configured to maintain the particles (12) in said fractionation microchannel (2), the fractionation microchannel being superimposed on the auxiliary microchannel (3), wherein the device (1) comprises two layers (19), each layer being with a microfabricated recess (14) which thickness (t) is less than 100 µm, the membrane (10) being mechanically held by encapsulation in between the two layers (19), the recesses (14) respectively defining the fractionation microchannel (2) and the auxiliary microchannel (3) on each side of the membrane (10).