公开(公告)号：EP1809651A4

公开(公告)日：2007-10-24

申请号：EP05799165

申请日：2005-10-21

申请人： AUSTRALIAN WOOL INNOVATION LTD

发明人： CRANSTON ROBIN WILLIAM ,  POOLE JACINTA MAREE ,  HICKEY MARK BRENDAN

IPC分类号： C07K1/107 ,  C07K1/14 ,  C07K2/00 ,  C07K14/435 ,  C08H1/06

CPC分类号： C08H1/06 ,  C07K14/4741

摘要： A process for separating keratinous proteins from a keratin-containing material, the process comprising the steps of: subjecting the keratin-containing material to reduction in a liquid medium to solubilise the keratinous proteins under conditions that minimise hydrolysis of the keratinous proteins, to yield a solution of keratinous proteins and undissolved solids; subjecting the solution of keratinous proteins to peroxide oxidation, without any intervening keratin precipitation step; and separating the solution of keratinous proteins from the undissolved solids prior to, at the same time as, or following the oxidation step. Preferred conditions for performing the reduction step involve contacting the keratin-containing material with a solution of an alkali metal sulfide reducing agent at a temperature of between 25 C and 50 C for a time of between 30 and 90 minutes, assuming atmospheric pressure. The peroxide oxidation is suitably carried out within not more than 4 hours after the reduction step, and involves reducing the pH of the solution to a level not less than pH 10, although pH 11.3 is most preferred. The product is demonstrated to have a principal fraction that has a molecular weight above 10 kDa, reflecting that the disulfide bonds in the keratinous proteins are broken without hydrolysis of the proteins.

摘要翻译： 一种从含角蛋白材料分离角质蛋白的方法，该方法包括以下步骤：使含角蛋白材料在液体介质中还原以在角蛋白水解最小化的条件下溶解角蛋白，得到 角质蛋白和不溶解固体的溶液; 使角蛋白溶液经受过氧化物氧化，而不需要任何介入的角蛋白沉淀步骤; 并在氧化步骤之前，同时或之后将角蛋白溶液与不溶解的固体分离。 进行还原步骤的优选条件包括使含角蛋白的材料与碱金属硫化物还原剂的溶液在25℃和50℃之间的温度下接触30-90分钟，假设大气压。 过氧化物氧化适宜在还原步骤后不超过4小时内进行，并且包括将溶液的pH降低至不低于pH10的水平，尽管pH11.3是最优选的。 证明该产物具有分子量高于10kDa的主要部分，这反映出角蛋白中的二硫键被破坏而不会蛋白质水解。