公开(公告)号：EP2564198B1

公开(公告)日：2017-01-25

申请号：EP11717447.4

申请日：2011-04-27

申请人： Abbott Point Of Care, Inc.

发明人： CAMPBELL, John Lewis Emerson ,  DAVIS, Graham ,  OMAKOR, John Emegbero ,  MOSS, Adam Roger ,  GUHADOS, Ganesh

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/74

CPC分类号： G01N33/54393 ,  G01N33/5306 ,  G01N33/5375 ,  G01N33/74 ,  G01N2333/475 ,  Y10T436/25125 ,  Y10T436/25375

公开(公告)号：EP2564198A1

公开(公告)日：2013-03-06

申请号：EP11717447.4

申请日：2011-04-27

申请人： Abbott Point Of Care, Inc.

发明人： CAMPBELL, John Lewis Emerson ,  DAVIS, Graham ,  OMAKOR, John Emegbero ,  MOSS, Adam Roger ,  GUHADOS, Ganesh

IPC分类号： G01N33/569

CPC分类号： G01N33/54393 ,  G01N33/5306 ,  G01N33/5375 ,  G01N33/74 ,  G01N2333/475 ,  Y10T436/25125 ,  Y10T436/25375

摘要： Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample such as a whole blood sample with one or more leukocidal reagents that reduce or eliminate the metabolic activity of leukocytes, and performing an immunoassay on the amended sample to determine the concentration of analyte in the sample. Preferably, the sample is amended with one or more enzymes and optionally one or more enzyme substrates and cofactors.

摘要翻译： 提供了用于减少分析物免疫测定中白细胞干扰的方法和装置。 在一个实施方案中，提供了一种方法，其包括用一种或多种减少或消除白细胞代谢活性的杀白细胞试剂修饰生物样品如全血样品的步骤，并对修改的样品进行免疫测定以确定浓度 样品中的分析物。 优选地，样品用一种或多种酶和任选的一种或多种酶底物和辅因子进行修饰。