"> PROTECTING GROUPS FOR
    2.
    发明公开
    PROTECTING GROUPS FOR "Z NUCLEOTIDE" AND METHODS THEREOF 审中-公开
    SCHUTZGRUPPENFÜRZ-NUKLEOTID UND VERFAHRENDAFÜR

    公开(公告)号:EP3154996A2

    公开(公告)日:2017-04-19

    申请号:EP15807404.7

    申请日:2015-05-28

    IPC分类号: C07H7/06

    摘要: Methods for synthesizing an oligonucleotide are provided in which the subject compounds find use. The method can include protecting an unnatural (e.g., Z) nucleotide with an acetyl-oxy-methyl group; incorporating the protected unnatural nucleotide into a nucleotide sequence on a solid support; and removing the acetyl-oxy-methyl group from the unnatural nucleotide incorporated into the nucleotide sequence. The compounds and methods find use in the synthesis of long oligonucleotides including Z nucleotides.

    摘要翻译: 本公开提供了独特的方法和保护基团,其能够以高产率以易于,清洁和位点特异性的方式合成具有Z核苷酸的寡核苷酸。 特别地,该方法对寡核苷酸产物几乎不产生损伤,并且不改变Z核苷酸本身。 本发明提供了有效合成具有Z核苷酸的长寡核苷酸的可行保护策略。