公开(公告)号：EP1015643B1

公开(公告)日：2005-09-21

申请号：EP99935503.5

申请日：1999-07-09

申请人： Agilent Technologies, Inc. (a Delaware corporation)

发明人： SAMPSON, Jeffrey, R. ,  YAKHINI, Zohar, H. ,  SAMPAS, Nicholas, M. ,  TSALENKO, Anna M., Apartment 811 ,  MYERSON, Joel ,  WEBB, Peter, G.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6872 ,  B01J2219/00454 ,  B01J2219/00497 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/00 ,  C12Q2537/143 ,  C12Q2533/101 ,  C12Q2533/107

摘要： Methods and reagents are disclosed which satisfy the need for more sensitive, more accurate and higher through-put analyses of target nucleic acid sequences. The methods and reagents may be generically applied to generally any target nucleic acid sequence and do not require a priori information about the presence, location or identity of mutations in the target nucleic acid sequence. The reagents of the invention are mixtures of natural and mass-modified oligonucleotide precursors having a high level of coverage and mass number complexity. A method is also disclosed for analyzing a target nucleic acid sequence employing the mixtures of natural and mass-modified oligonucleotide precursors and chemical or enzymatic assays to alter the mass of the oligonucleotide precursors prior to mass spectral analysis, generally via MALDI-TOF. The enzymatic assay may be a polymerase extension assay or a ligase assay. The kits for carrying out the methods of the invention are also disclosed.