公开(公告)号：EP2292748A1

公开(公告)日：2011-03-09

申请号：EP10185166.5

申请日：2004-10-12

申请人： AstraZeneca AB

发明人： Fenge, Christel ,  Lovgren, Ann ,  Thelin, Anders

IPC分类号： C12N9/64 ,  C12N9/74 ,  C12P21/02

CPC分类号： C12N9/647 ,  A61K38/00 ,  C12N9/6429 ,  C12P21/02 ,  C12Y304/21005

摘要： The present invention relates to methods and tools for producing large quantities of gamma-carboxylated protein comprising: (i) culturing a cell adapted to express a protein which requires gamma-carboxylation and γ-glutamyl carboxylase in a ratio of at least 10:1, under conditions suitable for expression of both proteins, and (ii) isolating gamma-carboxylated protein

摘要翻译： 本发明涉及用于生产大量γ-羧化蛋白质的方法和工具，其包括：（i）培养适于表达需要γ-羧化的蛋白质的细胞和至少10:1的比例的3-谷氨酰羧化酶， 在适合表达两种蛋白质的条件下，和（ii）分离γ-羧化蛋白质