专利检索 ap:("Aventis Pharma S.A.") AND inv:"REY, Lucien" 第 1 页

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发明公开
PROCEDE DE PURIFICATION DE FACTEUR DE STIMULATION DE COLONIES DE GRANULOCYTES 审中-公开
标题翻译：清理方法粒细胞集落刺激因子（G-CSF）

公开(公告)号：EP1200471A1

公开(公告)日：2002-05-02

申请号：EP00949647.2

申请日：2000-07-06

申请人： Aventis Pharma S.A.

发明人： DUMAS, Jacques , REY, Lucien , SARUBBI, Edoardo

IPC分类号： C07K14/535 , C07K1/20

CPC分类号： C07K14/535

摘要： The invention concerns a method for purifying G-CSF from a biological sample comprising steps which consist in: a) reducing the volume of the biological sample containing the G-CSF by hydrophobic interaction chromatography to obtain a concentrated, desalted and enriched fraction; b) passing the concentrated fraction over hydroxyapatite in conditions whereby the G-CSF is slightly bound to obtain a concentrated, desalted and enriched fraction containing the G-CSF; and c) collecting the G-CSF.