公开(公告)号：EP3266800A1

公开(公告)日：2018-01-10

申请号：EP17184307.1

申请日：2012-01-31

申请人： BAC IP B.V.

发明人： Hermans, Wilhelmus Josephus Johanna ,  Blokland, Sven ,  van Kesteren, Maria Anna ,  Horrevoets, Johanna Magaretha ,  Detmers, Franciscus Johan Marinus

IPC分类号： C07K16/42 ,  C07K16/06 ,  C07K1/22

CPC分类号： C07K16/4241 ,  C07K1/22 ,  C07K16/065 ,  C07K16/42 ,  C07K16/4283 ,  C07K2317/22 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/522 ,  C07K2317/569

摘要： The present disclosure relates to a method for the purification of a human IgG-CH1 domain comprising molecule using an antigen-binding protein that is capable of binding to an epitope that is comprised in the CH1 domain of each of human IgG1, human IgG2, human IgG3 and human IgG4. The disclosure further relates to the antigen-binding proteins that can be used in the method of the disclosure. The frame-work regions of the antigen-binding proteins of the disclosure preferably correspond to those of antibodies naturally that are devoid of light chains as may e.g. be found in camelids. The disclosure further relates to nucleic acids that encode such antigen-binding proteins, to immunoadsorbent materials that comprise such proteins, and to the uses of such immunoadsorbent materials for the purification of IgG-CH1 domain containing molecules from a variety of species.

摘要翻译： 本公开内容涉及使用抗原结合蛋白来纯化包含分子的人IgG-CH1结构域的方法，所述抗原结合蛋白能够结合包含在人IgG1，人IgG2，人类的每一个的CH1结构域中的表位 IgG3和人IgG4。 本公开还涉及可用于本公开方法中的抗原结合蛋白。 本公开的抗原结合蛋白的框架区优选对应于天然抗体的框架区，所述天然抗体不含轻链， 被发现在camelids。 本公开还涉及编码此类抗原结合蛋白的核酸，涉及包含此类蛋白的免疫吸附材料，并涉及此类免疫吸附材料用于纯化来自多种物种的含IgG-CH1结构域的分子的用途。