公开(公告)号：EP0206757A1

公开(公告)日：1986-12-30

申请号：EP86304700

申请日：1986-06-18

申请人： BENZON AS ALFRED

发明人： MOLIN SOREN ,  GERDES KENN AXO ,  RASMUSSEN POUL BAAD ,  ANDERSSON POUL KIRKETERP

IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/67 ,  C12N15/68

CPC分类号： C12N15/68 ,  C12N15/67 ,  Y10S435/874 ,  Y10S435/88

摘要： The stable maintenance of a replicon in a population of growing cells is ensured by providing the replicon with a sequence which encodes a product capable of killing the cell harbouring the replicon or the progeny of the cell (or encodes a precursor for the product) and a sequence encoding an antagonist for the killing product (or a precursor for the antagonist). The antagonist is one which suppresses the killing product (or a precursor for the killing product) in cells harbouring the replicon, whereas the antagonist activity decays when the replicon is lost from the cell so that the antagonist (or its precursor) is no longer continuously expressed. This means that the killing product (or its precursor) present in the now replicon-free cell is no longer suppressed by the antagonist, resulting in cell death. Cells containing the thus stabilized replicon may be grown on a large scale without any significant loss of the replicon from the cell population even when no selection pressure is applied.

摘要翻译： 通过向复制子提供编码能够杀死携带有细胞的复制子或后代的细胞的产物（或编码产物的前体）的序列，确保复制子在生长细胞群中的稳定维持，以及 编码杀死产物（或拮抗剂前体）的拮抗剂的序列。 拮抗剂是抑制在复制子的细胞中杀死产物（或杀死产物的前体）的拮抗剂，而当复制子从细胞中丢失时，拮抗剂活性衰减，使得拮抗剂（或其前体）不再连续 表达。 这意味着现在无复制子的细胞中存在的杀死产物（或其前体）不再被拮抗剂抑制，导致细胞死亡。 即使没有施加选择压力，含有如此稳定的复制子的细胞可以大规模生长，即使没有选择压力也不会从细胞群中显着损失复制子。