-
公开(公告)号：EP3848473A1
公开(公告)日：2021-07-14
申请号：EP18932607.7
申请日：2018-09-05
申请人： BGI Shenzhen
发明人： YANG, Xi , WANG, Wenjing , XING, Yanru , XU, Jinjin , CHEN, Fang , ZHU, Shida
摘要： Provided are an RNA library construction method and a specialized kit thereof. The method for preparing an RNA library includes the following steps: extracting RNA and performing fragmentation; adding a tail to 3' end; ligating an adaptor to 5' end and hybridizing with a DNA probe mixture; the DNA probe mixture is composed of several DNA probes that are reverse complementary to an RNA that is expected to be removed; removing RNA from the hybrid and removing DNA; performing reverse transcription and PCR amplification to obtain a library solution. The present invention combines poly A tailing and 5'end ligation. The RNA content in the system can be quickly increased by polyA tailing, thereby avoiding subsequent purification losses.
-
公开(公告)号：EP3988666A1
公开(公告)日：2022-04-27
申请号：EP19934021.7
申请日：2019-06-20
申请人： BGI Shenzhen
发明人： YANG, Xi , XING, Yanru , WANG, Wenjing
摘要： Provided are a method for constructing a library based on an RNA sample and uses thereof. The method includes: step 1 of subjecting the RNA sample to a reverse transcription reaction to obtain DNA-RNA hybrid strands; step 2 of performing reaction of the DNA-RNA hybrid strands with an endoribonuclease, a first DNA polymerase, a second DNA polymerase, and dATPs to obtain a double-stranded DNA added with dA-tail, where the first DNA polymerase has a 5'-3' exonuclease activity and a 3'-5' exonuclease activity, and the second DNA polymerase has no 3'-5' exonuclease activity; step 3 of ligating the double-stranded DNA added with dA-tail and a sequencing adaptor to obtain a ligated product; and step 4 of subjecting the ligated product to PCR amplification to obtain a sequencing library.
-
搜索结果
2 条记录 (耗时 0.006 秒)
