公开(公告)号：EP0925504B1

公开(公告)日：2001-03-14

申请号：EP97919095.6

申请日：1997-09-12

申请人： BIO MERIEUX

发明人： MALCUS-VOCANSON, Carine ,  PERRON, Hervé ,  MANDRAND, Bernard

IPC分类号： G01N33/564 ,  G01N33/50

CPC分类号： G01N33/564 ,  G01N33/5014 ,  G01N33/6896

公开(公告)号：EP0925504A1

公开(公告)日：1999-06-30

申请号：EP97919095.0

申请日：1997-09-12

申请人： BIO MERIEUX

发明人： MALCUS-VOCANSON, Carine ,  PERRON, Hervé ,  MANDRAND, Bernard

IPC分类号： C12Q1 ,  G01N33

CPC分类号： G01N33/564 ,  G01N33/5014 ,  G01N33/6896

摘要： The invention proposes a method for detecting and/or quantifying, in a biological sample a cytotoxic factor, in particular a gliotoxic factor, with respect to adherent target cells, in particular macroglial cells, the toxicity of which causes the death by apoptosis of said cells. The method consists in providing an initial fraction of said sample, optionally enriched with said toxic factor by previous treatment, incubating said initial toxic factor with a reference culture medium comprising adherent target cells, and detecting and/or quantifying the adherent target cells killed by apoptosis, by flux cytometry, at least one direct or indirect characteristic associated with the apoptotic adherent cells of the whole or part of the incubated medium, which, if it is present and/or is quantified, qualifies the sample as positive, i.e. as containing said toxic factor. The initial biological sample is preferably a urine specimen.