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1.发明公开Microwave method for preparation of serum samples for b-12 and folate assays 失效一种用于制备样品的血清维生素B12和使用微波叶酸分析过程。
公开(公告)号：EP0382334A2
公开(公告)日：1990-08-16
申请号：EP90300122.0
申请日：1990-01-05
IPC分类号： G01N33/82
CPC分类号： G01N33/5306 , G01N33/82
摘要： The present invention provides novel processes for the release of vitamin B₁₂ and/or folate in mammalian serum samples from endogenous serum binders comprising microwaving the serum samples prior to assaying the serum samples for vitamin B₁₂ and/or folate. Prior to the assay procedure, the serum samples are typically microwaved for about 20-90 seconds, most preferably for about 60 seconds at a high setting or such that the temperature of the serum samples is brought to a temperature of about 65 to 100°C, most preferably 80-85°C, for at least 15 seconds.
摘要翻译： 本发明提供了用于维生素B12和/或叶酸哺乳动物血清样品中释放内源性血清粘结剂以测定血清样品为维生素B12和/或叶酸包括微波血清样品之前的新方法。 在此之前的测定程序中，血清样品通常微波处理约20-90秒钟，最优选约60秒以高设置或检验没有血清样品的温度达到约65至100℃的温度下 ，最优选80-85℃，持续至少15秒。
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公开(公告)号：EP0348174A3
公开(公告)日：1991-05-22
申请号：EP89306264.6
申请日：1989-06-21
IPC分类号： G01N33/53 , G01N33/546
CPC分类号： G01N33/54313 , G01N33/5308 , G01N33/56966
摘要： The present invention relates to compositions and methods for the detection of sperm antibodies bound to the surface of spermatozoa or present in human serum, seminal plasma or cervical mucous through the use of immunoassays employing latex beads coupled to anti-human immunoglobulins. Preferably the beads are monodispersed polystyrene beads coupled to the anti-human immunoglobulins through a bridge of a polyamine, such as polylysine, having amino acid residues of about 200 to about 1,000. The beads are also preferably about 2 to about 4 microns in size.
摘要翻译： 本发明涉及通过使用使用与抗人免疫球蛋白偶合的乳胶珠的免疫测定来检测结合精子表面或存在于人血清，精浆或宫颈粘液中的精子抗体的组合物和方法。 优选地，珠粒是通过多胺（例如聚赖氨酸）的桥耦合到抗人免疫球蛋白的单分散聚苯乙烯珠，其氨基酸残基为约200至约1,000。 珠粒的尺寸也优选为约2至约4微米。
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3.发明公开
公开(公告)号：EP0337082A1
公开(公告)日：1989-10-18
申请号：EP89102828.4
申请日：1989-02-18
IPC分类号： G01N33/543 , G01N33/74 , G01N33/532 , G01N33/82
CPC分类号： G01N33/543 , G01N33/74
摘要： An immunoassay utilizing a biotin bridge is performed with a solid phase with anti-biotin immobilized on its surface. In accordance with the assay, the solid phase is incubated with a biotinylated binding species, preferably biotinylated antibody, specific for the ligand to be detected. This is then reacted with the test sample, followed by a labeled binding member, either in a sandwich-type or competitive-type binding assay. The label is then detected in accordance with conventional detection techniques. Use of the solid phase with only a biotin-specific antibody attached thereto expands the utility and versatility of a single solid phase for use in the detection of an unlimited variety of ligands.
摘要翻译： 使用生物素桥的免疫测定是在其表面固定有抗生物素的固相进行的。 根据该测定，将固相与待检测配体特异的生物素化结合物质，优选生物素化抗体孵育。 然后将其与测试样品反应，然后与夹心型或竞争型结合测定法中的标记结合成员反应。 然后根据常规检测技术检测标签。 仅使用与固定相结合的生物素特异性抗体的固相扩增了用于检测无限多种配体的单一固相的效用和通用性。
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4.发明公开Microwave method for preparation of serum samples for b-12 and folate assays 失效用于制备B-12和FOLATE测定的血清样品的MICROWAVE方法
公开(公告)号：EP0382334A3
公开(公告)日：1991-09-18
申请号：EP90300122.0
申请日：1990-01-05
IPC分类号： G01N33/82
CPC分类号： G01N33/5306 , G01N33/82
摘要： The present invention provides novel processes for the release of vitamin B₁₂ and/or folate in mammalian serum samples from endogenous serum binders comprising microwaving the serum samples prior to assaying the serum samples for vitamin B₁₂ and/or folate. Prior to the assay procedure, the serum samples are typically microwaved for about 20-90 seconds, most preferably for about 60 seconds at a high setting or such that the temperature of the serum samples is brought to a temperature of about 65 to 100°C, most preferably 80-85°C, for at least 15 seconds.
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公开(公告)号：EP0348174A2
公开(公告)日：1989-12-27
申请号：EP89306264.6
申请日：1989-06-21
IPC分类号： G01N33/53 , G01N33/546
CPC分类号： G01N33/54313 , G01N33/5308 , G01N33/56966
摘要： The present invention relates to compositions and methods for the detection of sperm antibodies bound to the surface of spermatozoa or present in human serum, seminal plasma or cervical mucous through the use of immunoassays employing latex beads coupled to anti-human immunoglobulins. Preferably the beads are monodispersed polystyrene beads coupled to the anti-human immunoglobulins through a bridge of a polyamine, such as polylysine, having amino acid residues of about 200 to about 1,000. The beads are also preferably about 2 to about 4 microns in size.
摘要翻译： 本发明涉及通过使用使用与抗人免疫球蛋白偶合的乳胶珠的免疫测定来检测结合精子表面或存在于人血清，精浆或宫颈粘液中的精子抗体的组合物和方法。 优选地，珠粒是通过多胺（例如聚赖氨酸）的桥耦合到抗人免疫球蛋白的单分散聚苯乙烯珠，其氨基酸残基为约200至约1,000。 珠粒的尺寸也优选为约2至约4微米。
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