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1.发明公开IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS 失效用于从细菌或真核细胞中纯化重组铜/锌（CU-ZN）超氧化物歧化酶的改进方法
公开(公告)号：EP0499621A4
公开(公告)日：1992-12-09
申请号：EP90917624
申请日：1990-11-07
发明人： BARTFELD, DANIEL , LIFSHITZ, RUTH , HADARY, DANY
IPC分类号： C12N9/02
CPC分类号： C12N9/0089
摘要： The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the biological activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof. The invention also provides a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog.
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