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1.发明公开METHOD FOR THE REMOVAL OF UNDESIRED CELLS FROM HUMAN LYMPHOCYTE POPULATIONS, APPLICATION OF THE METHOD IN MONOCLONAL ANTIBODY PRODUCTION AND KIT THEREFOR 失效从人类淋巴细胞群中去除有害细胞的方法，该方法在单克隆抗体生产中的应用和试剂盒
公开(公告)号：EP0342188A1
公开(公告)日：1989-11-23
申请号：EP87906018.0
申请日：1987-09-01
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2.发明授权METHOD FOR THE REMOVAL OF UNDESIRED CELLS FROM HUMAN LYMPHOCYTE POPULATIONS, APPLICATION OF THE METHOD IN MONOCLONAL ANTIBODY PRODUCTION AND KIT THEREFOR 失效从人类淋巴细胞群体中去除未细胞细胞的方法，该方法在单克隆抗体生产中的应用及其应用
公开(公告)号：EP0342188B1
公开(公告)日：1993-07-21
申请号：EP87906018.4
申请日：1987-09-01
CPC分类号： C12N5/0087 , C12N5/163
摘要： A method for the removal of, for in vitro immunisation, undesired cells from human lymphocyte containing cell populations. For the method, use is made of lysosomotropic agents which are caused to act in vitro on human lymphocyte containing cell populations. Also disclosed is a method in the in vitro immunisation of lymphocytes for use in the production of human monoclonal antibodies. In this method, lysosomotropic agents are caused to act in vitro on human lymphocyte containing cell populations for removing cell populations having a negative effect on the in vitro immunisation, whereupon the lymphocytes are in vitro immunised antigen-specifically and immortalised. Finally, a kit for in vitro immunisation of lymphocytes for use in the production of human monoclonal antibodies is disclosed. The kit comprises as active constituents a container containing lymphokines and a container containing lysosomotropic agents.
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