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公开(公告)号:EP0690927A1
公开(公告)日:1996-01-10
申请号:EP94906050.0
申请日:1994-01-06
发明人: FETTERHOFF, Terry J.
CPC分类号: C12Q1/6841 , C12Q1/6816 , G01N33/5094 , G01N33/56966
摘要: A method for fluorescent discrimination of cells that are nonviable prior to lysis or fixation techniques using a complementary pair of nucleic acid dyes which competitively bind to nucleic acid but which differ in their fluorescent emission characteristics. An especially preferred pair of dyes is 7-aminoactinomycin D (7-AAD) and actinomycin D (AD).