公开(公告)号：EP0789032A3

公开(公告)日：1997-08-20

申请号：EP97100856.0

申请日：1997-01-21

申请人： Bayer Corporation

发明人： Zhou, Zeqi ,  Armstrong E. Glenn Jr. ,  Yeung, Kwok K.

IPC分类号： C07K16/40 ,  C07K16/38 ,  G01N33/573 ,  G01N33/53

CPC分类号： G01N33/57434 ,  C07K16/40

摘要： A method for preparing a monoclonal antibody which, when used in a monoclonal/polyclonal antibody sandwich immunoassay method for measuring an analyte which can exist in both free and binding protein complexed forms in a biological test sample, provides an equimolar response to the free and complexed forms of the analyte. The monoclonal antibody is selected to have the properties of (i) upon binding to the analyte, rendering the analyte substantially incapable of binding with antibodies that can bind the free form, but not the complexed form, of the analyte, and (ii) binding substantially equivalently with free and complexed analyte. The method is particularly useful in the measurement of prostate-specific antigen (PSA) in blood, serum or plasma.

摘要翻译： 用于制备单克隆抗体的方法，当用于测量在生物测试样品中以游离和结合蛋白复合形式存在的分析物的单克隆/多克隆抗体夹心免疫测定方法时，提供对游离和复合的等摩尔响应 分析物的形式。 选择单克隆抗体以具有以下性质：（i）在与分析物结合后，使分析物基本上不能与能够结合分析物的游离形式而不是复合形式的抗体结合，和（ii）结合 基本上等同于游离和复合的分析物。 该方法在测量血液，血清或血浆中的前列腺特异性抗原（PSA）中特别有用。

公开(公告)号：EP0789032A2

公开(公告)日：1997-08-13

申请号：EP97100856.0

申请日：1997-01-21

申请人： Bayer Corporation

发明人： Zhou, Zeqi ,  Armstrong E. Glenn Jr. ,  Yeung, Kwok K.

IPC分类号： C07K16/40 ,  C07K16/38 ,  G01N33/573 ,  G01N33/53

CPC分类号： G01N33/57434 ,  C07K16/40

摘要： A method for preparing a monoclonal antibody which, when used in a monoclonal/polyclonal antibody sandwich immunoassay method for measuring an analyte which can exist in both free and binding protein complexed forms in a biological test sample, provides an equimolar response to the free and complexed forms of the analyte. The monoclonal antibody is selected to have the properties of (i) upon binding to the analyte, rendering the analyte substantially incapable of binding with antibodies that can bind the free form, but not the complexed form, of the analyte, and (ii) binding substantially equivalently with free and complexed analyte. The method is particularly useful in the measurement of prostate-specific antigen (PSA) in blood, serum or plasma.

摘要翻译： 一种用于制备单克隆抗体的方法，该方法，当在单克隆/多克隆抗体夹心免疫测定方法，用于同时可在自由和生物测试样品中结合蛋白复合的形式，提供在对游离和复合等摩尔响应存在的分析物的测定中使用 分析物的形式。 单克隆抗体被选择在结合于分析物，使该分析物基本上不能与抗体结合也能够结合游离形式为具有（i）所述的特性，而不是复合形式，分析物，和（ii）的结合 实质上等同自由和络合分析。 该方法是在血液，血清或血浆前列腺特异性抗原（PSA）的测量是特别有用的。