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    Method and apparatus for detection of microorganisms
    1.
    发明公开
    Method and apparatus for detection of microorganisms 有权
    Verfahren und Vorrichtung zum Nachweis von Mikroorganismen

    公开(公告)号：EP2980588A1

    公开(公告)日：2016-02-03

    申请号：EP14382299.7

    申请日：2014-07-31

    申请人： Betelgeux, S. L. ,  Photek Limited ,  Société 40-30

    发明人： Orihuel Iranzo, Enrique ,  Lorenzo Carton, Fernando ,  Ingle, Martin ,  Sundararajan, Shobitha ,  Blanc, Séverine ,  Hury, Stéphane ,  Giao, Maria S. ,  Keevil, Charles W. ,  Gilmartin, Niamh ,  O'Kennedy, Richard ,  Belenguer Ballester, José ,  Porta Banderas, Sonia

    IPC分类号： G01N33/569

    CPC分类号： G01N33/569 ,  G01N33/54326 ,  G01N2469/10

    摘要： The present invention is a method and an apparatus for identification and rapid detection of microorganisms, preferably grown on hard surfaces, by means of immunoassay techniques for separating the microorganism by magnetism and subsequently marking said separated microorganism with a fluorescent marker. Then, isolation and selection of microorganisms of a sample previously taken is based on an immunoassay reaction with microorganism-specific antibodies containing magnetic nanoparticles and fluorescent markers. Finally, detection and quantification of microorganism levels is achieved through excitation of the sample and measurement of luminescence by microorganism-antibodies conjugates. If there is any sampling, it is preferably performed through injection of pressurized air and water on a surface and recovery of microorganisms in a liquid sample.

    摘要翻译： 本发明是用于通过用于通过磁性分离微生物并随后用荧光标记物标记所述分离的微生物的免疫测定技术来鉴定和快速检测优选生长在硬表面上的微生物的方法和装置。 然后，先前采集的样品的微生物的分离和选择是基于与含有磁性纳米颗粒和荧光标记物的微生物特异性抗体的免疫测定反应。 最后，通过激发样品和通过微生物 - 抗体缀合物测量发光来实现微生物水平的检测和定量。 如果有任何取样，则优选通过在表面上注入加压空气和水并回收液体样品中的微生物来进行。