公开(公告)号：EP1373534B1

公开(公告)日：2005-12-21

申请号：EP02727959.5

申请日：2002-03-07

申请人： Biogemma ,  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA)

发明人： MARION, Didier ,  GAUTIER, Marie-Françoise ,  JOUDRIER, Philippe ,  MESSAGER, Arnaud ,  BARSBY, Tina ,  FREEMAN, Judy

IPC分类号： C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8245

摘要： A new method for increasing expression of a puroindoline protein in a plant, said puroindoline protein modifying grain hardness, notably breadmaking quality, said method comprising the steps of: (e) preparing a DNA construct which will allow the expression of a puroindoline protein, (f) introducing said DNA construct into plant cell, plant tissue, or plant, (g) selecting the plant cell, or plant tissue, or plant which stably maintain the puroindoline expression, (h) and optionally regenerating fertile stable transformed plant.

公开(公告)号：EP1373534A2

公开(公告)日：2004-01-02

申请号：EP02727959.5

申请日：2002-03-07

申请人： Biogemma ,  Institut National de la Recherche Agronomique (INRA)

发明人： MARION, Didier ,  GAUTIER, Marie-Françoise ,  JOUDRIER, Philippe ,  MESSAGER, Arnaud ,  BARSBY, Tina ,  FREEMAN, Judy

IPC分类号： C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8245

摘要： A new method for increasing expression of a puroindoline protein in a plant, said puroindoline protein modifying grain hardness, notably breadmaking quality, said method comprising the steps of: (e) preparing a DNA construct which will allow the expression of a puroindoline protein, (f) introducing said DNA construct into plant cell, plant tissue, or plant, (g) selecting the plant cell, or plant tissue, or plant which stably maintain the puroindoline expression, (h) and optionally regenerating fertile stable transformed plant.