专利检索 ap:("Biogen Idec MA Inc.") AND inv:"MIATKOWSKI, Konrad" 第 1 页

1.

发明授权
A METHOD FOR THE HIGH LEVEL EXPRESSION OF THE ACTIVE FORM OF LYMPHOTOXIN-BETA RECEPTOR IMMUNOGLOBULIN FUSION PROTEINS AND THEIR PURIFICATION 有权
标题翻译：方法为淋巴毒素β受体免疫球蛋白融合蛋白及其清洁活性形式的高表达

公开(公告)号：EP1141248B1

公开(公告)日：2011-12-07

申请号：EP99967350.2

申请日：1999-12-16

申请人： Biogen Idec MA Inc.

发明人： BROWNING, Jeffrey , MIATKOWSKI, Konrad , MEIER, Werner

IPC分类号： C07K14/705 , C07K19/00 , C12N5/10 , A61K38/17

CPC分类号： C12P21/00 , A61K38/00 , C07K14/70578 , C07K14/7151 , C07K2319/00 , C12P21/02

摘要： The invention relates to methods for the expression of high yields of the form protein-immunoglobulin fusions having high affinity binding to its ligand, by culturing hosts transformed with DNA encoding the desired fusions in a cultutre system at a low temperature thereby minimizing the amount of misfolded or misbridged protein forms. Said host cells may be transformed mammalian, insect, yeast or bacterial cells. The protein-immunoglobulin fusion may comprises a member of the TNF or TNF-receptor family, such as lymphotoxin-beta or lymphotoxin-beta receptor. The invention also relates to pharmaceutical composition comprising said protein-immunoglobulin fusions.