公开(公告)号：EP2524060A1

公开(公告)日：2012-11-21

申请号：EP11732315.4

申请日：2011-01-11

申请人： Biogen Idec MA Inc.

发明人： GORELIK, Leonid ,  SIMON, Kenneth, J. ,  SUBRAMANYAM, Meena ,  RUSHE, Mia, Marie

IPC分类号： C12Q1/70 ,  C12N15/86

CPC分类号： G01N33/56983 ,  G01N2333/025 ,  G01N2469/20 ,  G01N2800/50

摘要： The disclosure relates to methods and reagents for analyzing samples for the presence of JC virus antibodies. Disclosed is a method that includes obtaining a biological sample from a subject (e.g., plasma, serum, blood, urine, or cerebrospinal fluid), contacting the sample with highly purified viral-like particles (HPVLPs) under conditions suitable for binding of a JCV antibody in the sample to an HPVLP, and detecting the level of JCV antibody binding in the sample to HPVLP. In one embodiment, determining the level of anti-JCV antibodies in the subject sample provides a method of identifying PML risk in a subject.

摘要翻译： 本公开涉及用于分析样品存在JC病毒抗体的方法和试剂。 公开了一种方法，其包括从受试者（例如血浆，血清，血液，尿液或脑脊液）获得生物样品，在适合于JCV结合的条件下使样品与高度纯化的病毒样颗粒（HPVLP）接触 样品中的抗体与HPVLP的检测，并检测JCV抗体在样品中与HPVLP结合的水平。 在一个实施方案中，确定受试样品中抗JCV抗体的水平提供了鉴定受试者中PML风险的方法。