公开(公告)号：EP2943563A1

公开(公告)日：2015-11-18

申请号：EP14737761.8

申请日：2014-01-10

申请人： Biogen MA Inc.

发明人： GILBERT, Alan ,  MCELEARNEY, Kyle ,  DOBROWSKY, Terrence Michael ,  KSHIRSAGAR, Rashmi Rohit

IPC分类号： C12N5/00

CPC分类号： C12N5/0018 ,  C07K14/755 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/34 ,  C12N2511/00 ,  C12P21/00

摘要： The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.

公开(公告)号：EP2707383B1

公开(公告)日：2018-04-18

申请号：EP12723781.6

申请日：2012-05-11

申请人： Biogen MA Inc.

发明人： KSHIRSAGAR, Rashmi Rohit ,  GILBERT, Alan

IPC分类号： C07K1/113 ,  C12N5/00

CPC分类号： C12P21/00 ,  A61K39/39591 ,  A61K2039/505 ,  C07K1/1133 ,  C07K16/00 ,  C07K2317/40 ,  C07K2317/41 ,  C07K2317/94 ,  C12N5/0018 ,  C12N2500/34

摘要： The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.