公开(公告)号：EP2917873A1

公开(公告)日：2015-09-16

申请号：EP13788758.4

申请日：2013-11-07

申请人： Biomérieux ,  Commissariat à l'Énergie Atomique et aux Énergies Alternatives

发明人： ALLANO, Lorène ,  SUTRA, Guillaume ,  MUNOZ, Lorette ,  FULCHIRON, Corine ,  DECAUX, Dominique ,  PINSTON, Frédéric

IPC分类号： G06K9/00 ,  G06T7/00

CPC分类号： G06K9/0014 ,  G06K9/00127 ,  G06K9/00134 ,  G06T5/005 ,  G06T7/0012 ,  G06T7/12 ,  G06T7/73 ,  G06T7/90 ,  G06T2207/10016 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/10152 ,  G06T2207/20061 ,  G06T2207/20076 ,  G06T2207/20152 ,  G06T2207/30024 ,  G06T2207/30242

摘要： A method for defining an isolation area around an object of interest in a cell culture vessel, the method comprising the steps of: - obtaining one or more images of the cell culture vessel using one or more of a plurality of illumination sources, each illumination source being capable of illuminating the vessel from a different direction; - selecting an image or combination of images for further processing; - applying a circular object detection transformation to identify one or more objects of interest being substantially circular objects in the cell culture vessel, which circular objects of interest are representative of isolated colonies in the cell culture vessel and determining the center of an object of interest; - applying a binarizing step to obtain a binarized image of the object of interest and other objects, wherein the center of the binarized image corresponds to the center of the object of interest; - iteratively forming concentric circles with increasing radius, wherein the concentric circles are centered on the center of the binarized image; - identifying coronas, wherein a corona is delimited by two circles having successive radius values; - for each corona: determining the presence and the location of any other object located in the corona to determine the presence and the location of other objects; - determining a clearance angle defining an angular sector free of other objects around the object of interest to define the isolation area around the object of interest.

摘要翻译： 一种用于在细胞培养容器内定义感兴趣对象周围的隔离区域的方法，所述方法包括以下步骤：使用多个照明源中的一个或多个获得所述细胞培养容器的一个或多个图像，每个照明源 能够从不同的方向照射船只; - 选择图像或图像组合进行进一步处理; - 应用圆形物体检测变换来识别细胞培养容器中基本为圆形物体的一个或多个感兴趣对象，所述感兴趣的圆形物体代表细胞培养容器中的孤立菌落并确定感兴趣对象的中心; - 应用二值化步骤来获得感兴趣对象和其他对象的二值化图像，其中二值化图像的中心对应于感兴趣对象的中心; - 迭代地形成具有增加半径的同心圆，其中同心圆以二值化图像的中心为中心; - 识别冠状物，其中电晕由具有连续半径值的两个圆界定; - 对于每个电晕： - 确定位于电晕中的任何其它物体的存在和位置，以确定其它物体的存在和位置; - 确定间隙角度，其定义围绕感兴趣对象的其它物体的角扇区，以限定感兴趣对象周围的隔离区域。