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    Method of producing polyhydroxyalkanoate from alkane having residue containing aromatic ring in its molecule
    9.
    发明公开
    Method of producing polyhydroxyalkanoate from alkane having residue containing aromatic ring in its molecule 有权
    一种用于生产在分子中具有芳族环烷烃的聚羟基烷酸酯过程

    公开(公告)号：EP1367078A2

    公开(公告)日：2003-12-03

    申请号：EP03007890.1

    申请日：2003-04-07

    申请人： CANON KABUSHIKI KAISHA

    发明人： Kenmoku, Takashi, c/o Canon Kabushiki Kaisha ,  Sugawa, Etsuko, c/o Canon Kabushiki Kaisha ,  Yano, Tetsuya, c/o Canon Kabushiki Kaisha ,  Imamura, Takeshi, c/o Canon Kabushiki Kaisha ,  Honma, Tsutomu, c/o Canon Kabushiki Kaisha

    IPC分类号： C08G63/00 ,  C12P7/62

    CPC分类号： C08G63/6882 ,  C12P7/625 ,  C12P11/00

    摘要： The present invention provides a method of effectively producing polyhydroxyalkanoate having an aromatic substituted residue in its monomer unit. In the method of producing polyhydroxyalkanoate by using microorganisms, microorganisms capable of producing polyhydroxyalkanoate are cultured in a culture medium containing at least one starting compound selected from the group consisting of substituted alkanes represented by formula (1) to produce polyhydroxyalkanoate having, in its molecule, at least one unit selected from the group consisting of 3-hydroxy-substituted alkanoate units represented by formula (2):

            R―(CH 2 ) n ―CH 2 ―CH 2 ―CH 3      (1)

    wherein R represents a residue containing a substituted aromatic ring, and n represents any integer of 1 to 8;
    wherein R represents a residue containing a substituted aromatic ring, and n represents any integer of 1 to 8. In formulas (1) and (2), a residue R is as described in the specification.

    摘要翻译： 本发明提供了具有在其单体单元中的芳族残基substituiertem有效生产聚羟基烷酸酯的方法。 在制造聚羟基脂肪酸酯的，通过使用微生物的方法，能生产聚羟基烷酸酯的微生物在培养基中包含选自下组substituiertem烷烃中的至少一种起始化合物培养的由式（1），以产生具有聚羟基烷酸酯，在其分子为代表， 选自3-羟基链烷酸酯单元substituiertem由式（2）表示的至少一种单元：R-（CH 2）N-CH2-CH2-CH3 worinřdarstellt的残基包含一个substituiertem芳族环，且n darstellt 的1至8的任何整数; worinřdarstellt的残基包含一个substituiertem芳族环，且n darstellt〜8的1的任何整数。在式（1）和（2）的残基R是如在说明书中描述。

    Method and apparatus for producing polyhydroxyalkanoate
    10.
    发明公开
    Method and apparatus for producing polyhydroxyalkanoate 审中-公开
    Verfahren zur产品von Polyhydroxyalkanoat

    公开(公告)号：EP1245682A2

    公开(公告)日：2002-10-02

    申请号：EP02007011.6

    申请日：2002-03-27

    申请人： CANON KABUSHIKI KAISHA

    发明人： Honma, Tsutomu, c/o Canon Kabushiki Kaisha ,  Sugawa, Etsuko, c/o Canon Kabushiki Kaisha ,  Yano, Tetsuya, c/o Canon Kabushiki Kaisha ,  Nomoto, Tsuyoshi, c/o Canon Kabushiki Kaisha ,  Imamura, Takeshi, c/o Canon Kabushiki Kaisha ,  Suzuki, Tomohiro, c/o Canon Kabushiki Kaisha ,  Kenmoku, Takashi, c/o Canon Kabushiki Kaisha

    IPC分类号： C12P7/62 ,  C12M1/00

    CPC分类号： C12P7/625 ,  C12M47/06

    摘要： The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.

    摘要翻译： 本发明提供了通过从含有PHA的细胞中除去PHA以外的细胞成分而以高产率生产高纯度聚羟基链烷酸酯（PHA）的方法。 此外，本发明提供了如上所述在生产PHA中进行含有次氯酸盐的氧化剂的处理时，简单地除去或还原所收集的PHA颗粒中残留的氯的方法。 上述方法之一包括用至少含有次氯酸盐的氧化剂处理含有聚羟基链烷酸酯的细胞的步骤，将经处理的细胞分离成水溶性部分和水不溶性部分的步骤，以及还原氯 保留在水不溶性部分中。 还原氯的步骤可以是用至少含有聚羟基链烷酸酯不溶的有机极性溶剂的热水，硫代硫酸盐溶液或极性溶剂溶液洗涤水不溶性部分的步骤。