摘要:
A recombinant DNA is constructed by using a toluene monooxygenase gene isolated from Ralstonia eutropha strain TB64 and employed to provide the transformant which can express toluene monooxygenase useful for cleaning of aqueous media such as drain and waste water containing halogenated aliphatic hydrocarbon compounds or aromatic compounds, for remediation of soil polluted with such compounds, and cleaning of air (gas phase) polluted with volatile organic chlorine compounds.
摘要:
The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.
摘要:
To provide a structure that has both drug holding capability and sustained releasability a liposome and mechanical strength of polyhydroxyalkanoate, is excellent in holding capability for hydrophilic drugs and other watersoluble substances as well as liphophilic drugs and other hydrophobic substances, and is capable of controlling the sustained releasability. To coat with polyhydroxyalkanoate at least part of the outer wall of the liposome.
摘要:
A polyhydroxyalkanoate is disclosed which has in the molecule a unit represented by Chemical Formula (1). wherein R is arbitrarily selected from a hydrogen atom, a halogen atom, CN, NO 2 , COOR', SO 2 R", CH 3 , C 2 H 5 , C 3 H 7 , C(CH 3 ) 2 H and C(CH 3 ) 3 ; where R' is H, Na, K, CH 3 or C 2 H 5 , and R" is OH, ONa, OK, a halogen atom, OCH 3 or OC 2 H 5 ; and x is an integer arbitrarily selected from 1 to 8; with the proviso that a polyhydroxyalkanoate is excluded which has a hydrogen atom as R and x in all the units is 2 or 4. Also disclosed is a process for producing the polyhydroxyalkanoate by the use of a microorganism having the ability to produce the polyhydroxyalkanoate and accumulate it in the bacterial body.