公开(公告)号：EP3126485A4

公开(公告)日：2017-11-29

申请号：EP15774049

申请日：2015-03-31

申请人： CAPITALBIO CORP ,  UNIV TSINGHUA

发明人： LIU YAN ,  ZHANG GUOHAO ,  ZHU JIANG ,  FENG ZHENGDE ,  GUO HONGJU ,  GAO JIAN ,  XING WANLI ,  CHENG JING

IPC分类号： C12M1/34 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L3/502723 ,  B01L2200/10 ,  B01L2300/048 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/6844

摘要： In one aspect, the present disclosure provides an integrated microfluidic device for nucleic acid amplification and microarray detection. In one aspect, the device comprises: (1) a microchip configured to process reagents, comprising a plurality of reservoirs, channels, valves, and/or fluid interfaces; (2) an amplification chamber for PCR, carried out in a detachable tube assembled on the microchip through a joint; and (3) a microarray chamber comprising a microarray and a reaction chamber. In some embodiments, these features are interconnected to allow transportation of reagents for nucleic acid amplification and hybridization detection functions in a closed system. In one aspect, the integrate device herein overcomes the problem of contamination during the amplification and hybridization reactions.