公开(公告)号：EP1891446A1

公开(公告)日：2008-02-27

申请号：EP06763568.0

申请日：2006-06-07

申请人： CELLZOME AG

发明人： DREWES, Gerard ,  KUESTER, Bernhard ,  KRUSE, Ulrich ,  HOPF, Carsten ,  EBERHARD, Dirk ,  BANTSCHEFF, Marcus ,  READER, Valerie ,  MIDDLEMISS, David ,  RAIDA, Manfred

IPC分类号： G01N33/68 ,  C12Q1/48

CPC分类号： G01N33/6848 ,  C12Q1/485

摘要： The present invention relates to methods for the characterization of enzymes or of enzyme-compound complexes, wherein the enzyme is obtained from a protein preparation with the help of at least one broad spectrum ligand immobilized on a solid support and wherein the enzyme is characterized by mass spectrometry. These methods are useful for the screening of non-immobilized compound libraries, selectivity profiling of lead compounds and mechanism of action studies in living cells.