公开(公告)号：EP1046338A1

公开(公告)日：2000-10-25

申请号：EP00201779.6

申请日：1995-08-10

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA ,  Kambeel, Robert Wilhelmus

发明人： Mena Campos, Jesus ,  Fernandez Machuca, Marina ,  De la Riva de la Riva, Gustavo Alberto ,  Coego Gonzalez, Alberto ,  Vasquez Mendez, Ramon P. ,  Garcia Gonzalez, Melba ,  Pimentel Vasquez, Eulogio ,  Lopez Quesada, Alina ,  Garcia Gonzalez, Rolando ,  Zaldua Guerra, Zurima ,  Mencho Ponce, Juan Diego

IPC分类号： A01N63/00 ,  C12R1/15 ,  C12R1/01

CPC分类号： C12R1/15 ,  A01N63/00 ,  C12R1/01

摘要： New bacterial strains, in particular Sphingobacterium spiritivorum C-926 and Corynebacterium paurometabolum C-924 with useful characteristics in the farming sector due to their nematicidic effect, which can be used in this context for the bio-control of nematodes. Bionematicides capable of effectively controlling phytopathogenic nematode populations in various crops of interest. Useful strains may be isolated from soil taken from areas habitually affected by a high incidence of nematodes, from banana plants showing less severe symptoms of infestation. Strains are selected which are capable of affecting the eggs of the nematode Meloidogyne incognita, in experiments carried out under laboratory conditions. Active strains, such as Sphingobacterium spiritivorum strain C-926 and Corynebacterium paurometabolum strain C-924, are tested to assess the protection which the bacterial preparation, applied directly, can provide to pumpkin and banana plants in relation to Meloidogyne incognita and Radopholus similis. Strains may also be tested against the zoonematodes Haemonchus sp. and Trichostrongylus sp. Control of these parasites allows veterinary use. In addition to the nematodes referred to above, and selected due to the high losses which they incur in crops, the strains may also be effective in the control of populations of Heterodera ssp., Globodera ssp., and Pratylenchus ssp., as well as other species of zoonematodes.

摘要翻译： 获得具有杀线虫性的细菌菌株的方法包括从受线虫影响的区域收集土壤和/或植物组织的样品，将受影响较大的材料的样品与受影响较少的材料的样品区分开来，分离存在于所述样品中的细菌菌株，比较细菌 存在于受影响较小的样品中存在的菌株的菌株存在，选择仅在受影响较小的样品中存在的菌株，测试所选菌株的杀线虫性质并选择具有有用的杀线虫性质的那些菌株。 用于线虫生物防治的方法包括使土壤，植物或种子与有效量的杀线虫剂接触，所述杀线虫剂是由鞘氨醇灵菌C-926和/或原代梭菌代谢C-924获得的培养物或代谢物 衍生自所述菌株，通过天然，重组或合成途径在合适的载体中获得，或在兽医学中使用所述杀线虫剂。 用于控制300种线虫。 在这种方法中用作线虫剂的所述培养物，或其对源自线虫有活性的任何突变也是要求保护的，以及包含所述试剂和合适的载体的线性剂。