公开(公告)号：EP0966535A1

公开(公告)日：1999-12-29

申请号：EP98900843.0

申请日：1998-01-13

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB) ,  Instituto de Medicina Tropical "Pedro Kouri" IPK

发明人： VAZQUEZ RAMUDO, Susana ,  GUZMAN TIRADO, Guadalupe ,  GUILLEN NIETO, Gerardo Enrique ,  PARDO LAZO, Orlando Luis ,  CHINEA SANTIAGO, Glay ,  PEREZ DIAZ, Ana Beatriz ,  PUPO ANTUNEZ, Maritza ,  RODRIGUEZ ROCHE, Rosmari ,  REYES ACOSTA, Osvaldo ,  GARAY PEREZ, Hilda Elisa ,  PADRON PALOMARES, Gabriel ,  ALVAREZ VERA, Maylin ,  MORIER DIAZ, Luis ,  PEREZ INSUITA, Omaida ,  PELEGRINO MARTINEZ DE LA COTERA, José Luis

IPC分类号： C12N15 ,  A61K38 ,  A61K39 ,  A61P31 ,  C07K14 ,  C07K16

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24122 ,  G01N2333/18 ,  Y02A50/386

摘要： The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.

公开(公告)号：EP0966535B1

公开(公告)日：2004-12-08

申请号：EP98900843.8

申请日：1998-01-13

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB) ,  Instituto de Medicina Tropical "Pedro Kouri" IPK

发明人： VAZQUEZ RAMUDO, Susana ,  GUZMAN TIRADO, Guadalupe ,  GUILLEN NIETO, Gerardo Enrique ,  PARDO LAZO, Orlando Luis ,  CHINEA SANTIAGO, Glay ,  PEREZ DIAZ, Ana Beatriz ,  PUPO ANTUNEZ, Maritza ,  RODRIGUEZ ROCHE, Rosmari ,  REYES ACOSTA, Osvaldo ,  GARAY PEREZ, Hilda Elisa ,  PADRON PALOMARES, Gabriel ,  ALVAREZ VERA, Maylin ,  MORIER DIAZ, Luis ,  PEREZ INSUITA, Omaida ,  PELEGRINO MARTINEZ DE LA COTERA, José Luis

IPC分类号： C12N15/40 ,  C07K14/18 ,  A61K39/12 ,  C07K16/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24122 ,  G01N2333/18 ,  Y02A50/386

摘要： The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.