公开(公告)号：EP0820463A1

公开(公告)日：1998-01-28

申请号：EP96912727.0

申请日：1996-04-12

申请人： CEPHALON, INC.

发明人： BRIERLEY, Russell, A. ,  ABRAMS, Joan, N. ,  HANSON, John, M. ,  MASLANKA, Francis, C.

IPC分类号： C12N15 ,  C07K1 ,  C07K14 ,  C12P21 ,  C12R1 ,  G01N30

CPC分类号： C07K14/65 ,  G01N30/02 ,  B01D15/422

摘要： Disclosed is a process for obtaining purified, monomeric, intact, correctly-folded insulin-like growth factor-I (also known as somatomedin-C). The process includes the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step in order to increase the final yield. The process also includes the following steps in order: first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.

公开(公告)号：EP0820463B1

公开(公告)日：2008-11-26

申请号：EP96912727.3

申请日：1996-04-12

申请人： CEPHALON, INC. ,  Novartis Vaccines and Diagnostics, Inc.

发明人： BRIERLEY, Russell, A. ,  ABRAMS, Joan, N. ,  HANSON, John, M. ,  MASLANKA, Francis, C. ,  COWGILL, Cynthia ,  JUARBE-OSORIO, Luis G. ,  RIQUELME, Patricio ,  DORIN, Glenn

IPC分类号： C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/36

CPC分类号： C07K14/65 ,  G01N30/02 ,  B01D15/422

摘要： Disclosed is a process for obtaining purified, monomeric, intact, correctly-folded insulin-like growth factor-I (also known as somatomedin-C). The process includes the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step in order to increase the final yield. The process also includes the following steps in order: first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.