公开(公告)号：EP1921140A4

公开(公告)日：2009-05-06

申请号：EP06780756

申请日：2006-07-04

申请人： CHEMO SERO THERAPEUT RES INST

发明人： KAMINAKA KAZUYOSHI ,  MAEDA HIROAKI ,  HIRASHIMA MASAKI ,  KAWAMURA RYOICHI ,  MATSUDA JUNICHI ,  KUWANA TAKASHI

IPC分类号： C12N15/09 ,  A01K67/027 ,  C12N5/10

CPC分类号： C12N15/79 ,  A01K2217/05 ,  A01K2227/30 ,  C12N15/102 ,  C12N15/85 ,  C12N15/90 ,  C12N2800/30 ,  C12N2800/90 ,  C12N2820/00

摘要： Disclosed are a mutant transposon vector and a method for introducing a foreign gene into a cell. A mutant transposon vector having a nucleic acid fragment inserted therein, the nucleic acid fragment having the properties (i) and (ii) or the properties (i) to (iii) below; a foreign gene-expressing cell which is prepared by introducing the mutant transposon vector into the chromosomal DNA of an established cell or a cell capable of being individuated and then additionally introducing a foreign gene-expressing cassette into a recombinant system (e.g., Cre-Lox system); a recombinant animal created by using the cell (in the case where the cell capable of being individuated is used); and a method for producing a foreign protein from the cell or the recombinant animal: (i) comprising a 5'-TIR sequence and a 3'-TIR sequence of transposon gene; (ii) having a sequence at which a recombination reaction occurs inserted between at least one set of DR regions of two sets of DR regions present in a 5'-TIR sequence or a 3'-TIR sequence of transposon gene; and (iii) having a restriction recognition site or a foreign gene-expressing cassette inserted between a 5'-TIR sequence and a 3'-TIR sequence of transposon gene.

摘要翻译： 提供了修饰的转座子载体和将外源基因导入细胞的方法。 一种修饰的转座子载体，其中插入具有以下特征（a） - （b）或（a） - （c）的核酸片段; 通过将所述载体导入细胞系或本体细胞的染色体DNA而获得的表达外源基因的细胞，并进一步将外源基因的表达盒导入染色体DNA; 通过使用所述细胞产生的遗传重组的动物（在本体细胞的情况下）; 以及从所述细胞和所述遗传重组动物产生外源蛋白的方法：（a）由转座子基因的5'和3'-TIR序列组成; （b）发生重组反应的序列插入转座子基因的5'-和3-TIR序列中的两个存在的DR区域中的至少任一个之间; （c）在转座子基因的5'和3'-TIR序列之间插入限制酶识别位点或外来基因的表达盒。