公开(公告)号：EP2022849A4

公开(公告)日：2010-10-06

申请号：EP07743165

申请日：2007-05-11

申请人： CHEMO SERO THERAPEUT RES INST

发明人： NOUCHI TOSHINOBU ,  NISHIYAMA KIYOTO ,  SUGAWARA KEISHIN

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  A61K2039/525 ,  C12N2501/70 ,  C12N2760/16051

公开(公告)号：EP1939283A4

公开(公告)日：2009-02-25

申请号：EP06810820

申请日：2006-09-29

申请人： CHEMO SERO THERAPEUT RES INST

发明人： ONCHI TATSUFUMI ,  NAKAHARA YO ,  IMAMURA TAKAYUKI ,  NOUCHI TOSHINOBU ,  NAKATAKE HIROSHI

IPC分类号： C12N9/74 ,  C12N5/10 ,  C12N15/09

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： A process for producing a recombinant human thrombin. There is provided a process for producing a recombinant human thrombin, comprising the steps of (1) introducing an expression vector having a gene fragment coding for human prothrombin gene inserted therein in CHO cells to thereby obtain recombinant cells capable of producing human prothrombin; (2) isolating human prothrombin from a culture of the recombinant CHO cells according to anion exchange technique; (3) causing ekaline to act on the isolated human prothrombin to thereby convert the human prothrombin to human thrombin; and (4) isolating human thrombin from the ekaline treatment liquid in accordance with an affinity force based method in which use is made of benzamidine and cation exchange technique. Further, there are provided, obtained by the above process, human thrombin and human prothrombin producing CHO cell.