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公开(公告)号：EP1471073A2
公开(公告)日：2004-10-27
申请号：EP04076119.9
申请日：1991-11-07
申请人： CHIRON CORPORATION
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222
摘要： Two Hepatitis C Virus envelope proteins (El and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles. Cells bearing a mannose receptor or asialoglycoprotein receptor are capable of being infected with HCV, and supporting culturing of the virus.
摘要翻译： 以下要求：（A）选自E1和E2的分离的丙型肝炎病毒（HCV）唾液酸糖蛋白（AG）; （B）HCV AG的纯化方法，其包括（a）使化合物 contg。 具有甘露糖结合蛋白的HCV AG，例如。 刀豆豆素A（ConA）或天竺葵凝集素（GNA）和（b）分离组分的部分。 其结合甘露糖结合蛋白，例如 通过甘露糖洗脱; （c）用于检测HCV AG的存在的测定试剂盒，其包含（a）固体支持物，（b）甘露糖结合蛋白质和（c）对HCV AG特异的抗体，其中抗体和 甘露糖结合蛋白与固体支持物结合; （D）用于确定HCV感染或感染的方法，其中体液样品中的任何HCV都是一致的。 在测定之前通过与甘露糖结合蛋白接触; （E）用用于重组表达HCV AG的载体转化的细胞，其中所述载体包含编码糖基化信号的结构基因，HCV AG，在宿主细胞中可操作并且能调节HCV AG表达的调节序列 和选择标记，其中细胞不唾液酸化糖蛋白; 和（F）减少或消除血浆，血清或其他生物液体中HCV的存在的方法。
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公开(公告)号：EP0842947A3
公开(公告)日：2001-12-12
申请号：EP97120661.0
申请日：1991-11-07
申请人： CHIRON CORPORATION
IPC分类号： C07K14/18 , C12N15/40 , A61K39/29 , G01N33/576
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222
摘要： Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles. Cells bearing a mannose receptor or asialoglycoprotein receptor are capable of being infected with HCV, and supporting culturing of the virus.
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公开(公告)号：EP0842947A2
公开(公告)日：1998-05-20
申请号：EP97120661.0
申请日：1991-11-07
申请人： CHIRON CORPORATION
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222
摘要： Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles. Cells bearing a mannose receptor or asialoglycoprotein receptor are capable of being infected with HCV, and supporting culturing of the virus.
摘要翻译： 两种丙型肝炎病毒包膜蛋白（E1和E2）都不表达唾液酸化。 这些蛋白质在低等真核生物或其末端糖基化被阻断的哺乳动物细胞中的重组表达导致与天然HCV糖蛋白更相似的重组蛋白质。 当通过GNA凝集素亲和力分离时，E1和E2蛋白聚集成病毒样颗粒。 具有甘露糖受体或脱唾液酸糖蛋白受体的细胞能够感染HCV，并支持病毒的培养。
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公开(公告)号：EP1471073A3
公开(公告)日：2004-12-01
申请号：EP04076119.9
申请日：1991-11-07
申请人： CHIRON CORPORATION
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222
摘要： Two Hepatitis C Virus envelope proteins (El and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles. Cells bearing a mannose receptor or asialoglycoprotein receptor are capable of being infected with HCV, and supporting culturing of the virus.
摘要翻译： 两种丙型肝炎病毒包膜蛋白（E1和E2）在没有唾液酸化的情况下表达。 这些蛋白质在低等真核生物中或在终端糖基化被阻断的哺乳动物细胞中的重组表达导致与天然HCV糖蛋白更相似的重组蛋白质。 当通过GNA凝集素亲和力分离时，E1和E2蛋白聚集成病毒样颗粒。 带有甘露糖受体或脱唾液酸糖蛋白受体的细胞能够感染HCV，并支持病毒的培养。
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公开(公告)号：EP0842947B1
公开(公告)日：2004-04-21
申请号：EP97120661.0
申请日：1991-11-07
申请人： CHIRON CORPORATION
IPC分类号： C07K14/18 , C12N15/40 , A61K39/29 , G01N33/576
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222
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