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    PROCEDE DE DETECTION DE COMPOSES MODULATEURS DE DIMERES DE PROTEINES MEMBRANAIRES A DOMAINE VFT
    1.
    发明公开
    PROCEDE DE DETECTION DE COMPOSES MODULATEURS DE DIMERES DE PROTEINES MEMBRANAIRES A DOMAINE VFT 有权
    检测方法的VFT域膜蛋白调节性化合物的二聚体

    公开(公告)号：EP2425249A1

    公开(公告)日：2012-03-07

    申请号：EP10727062.1

    申请日：2010-04-29

    申请人： CIS Bio International ,  Centre National de la Recherche Scientifique

    发明人： DOUMAZANE, Etienne ,  ZWIER, Jurriaan ,  TRINQUET, Eric ,  PIN, Jean-Philippe

    IPC分类号： G01N33/542

    CPC分类号： G01N33/542 ,  G01N33/9426 ,  G01N2333/726 ,  G01N2500/04

    摘要： The invention relates to a method for selecting compounds having a modulating effect on the activation state of a dimer of VFT domain proteins expressed in cellular membranes in a measuring medium, said dimer including a first protein and a second protein which are identical or different, wherein the method includes the following steps: (a) marking the first and second proteins in the N-terminal portion of the VFT domain by members of a FRET partner pair, the Förster radius (R
    0 ) of said pair ranging between 20 and 55 Å; (b) measuring the FRET signal in the absence and in the presence of the compound to be tested within a predetermined time window; (c) selecting the compound to be tested as a modulating compound if a difference in the FRET signal in the absence and in the presence of the compound to be tested is measured during step (b). The invention can be used in the research for new drugs and new taste modulators.