公开(公告)号：EP1694703A1

公开(公告)日：2006-08-30

申请号：EP04808311.7

申请日：2004-12-04

申请人： CJ Corporation

发明人： PARK, Ji-Sook ,  CHUNG, Jong-Sang ,  BAEK, Min-Ji ,  AHN, Jee-Won ,  KIM, Ki-Wan ,  PARK, Hyung-Ki ,  LEE, Dong-Eok ,  OH, Myung-Suk

IPC分类号： C07K14/565

CPC分类号： C07K14/565 ,  C12P21/02

摘要： Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and cation exchange chromatography, wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt% of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt% of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt% of propylene glycol and 1-2M NaCl.

公开(公告)号：EP1697411A1

公开(公告)日：2006-09-06

申请号：EP04808310.9

申请日：2004-12-04

申请人： CJ Corporation

发明人： PARK, Ji-Sook ,  CHUNG, Jong-Sang ,  BAEK, Min-Ji ,  AHN, Jee-Won ,  KIM, Ki-Wan ,  PARK, Hyung-Ki ,  LEE, Dong-Eok ,  OH, Myung-Suk

IPC分类号： C07K14/565

CPC分类号： C07K14/565 ,  C07K5/06139

摘要： Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt% of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt% of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt% of propylene glycol and 1-2M NaCl.

摘要翻译： 本发明提供一种从含重组人干扰素β的培养物中纯化人干扰素β的方法，该方法包括进行亲和层析和反相高效液相色谱（RP-HPLC），其中亲和层析包括：吸附含干扰素β的培养物 平衡的亲和层析柱上，然后用平衡缓冲溶液洗涤; 用含有30-60wt％丙二醇的pH6.5-7.5的洗涤缓冲液A和含有10-30wt％丙二醇和1-2M NaCl的pH6.5-7.5的洗涤缓冲液B洗涤柱子; 并用含有40-60wt％丙二醇和1-2M NaCl的pH6.5-7.5的缓冲溶液洗脱含人干扰素β的级分。