公开(公告)号：EP0866855A4

公开(公告)日：2000-02-02

申请号：EP96937920

申请日：1996-11-15

申请人： COMMW SCIENT IND RES ORG

发明人： JACKSON RON

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N9/10 ,  C12N15/00 ,  C12N15/39 ,  C12R1/91 ,  C12R1/92 ,  C07K14/065

CPC分类号： C12N9/1081

摘要： A method of preparation of lysate having alpha 2,3-sialyltransferase activity is disclosed. A cell culture having essentially no endogenous expression of eukaryotic alpha 2,3-sialyltransferase is infected with myxoma virus. The harvested triton lysates include significant quantities of a protein having eukaryotic alpha 2,3-sialyltransferase activity. Compositions having in vitro alpha 2,3-sialyltransferase activity and comprising a protein extract of lysates of the aforementioned lysates are also provided for.