公开(公告)号：EP0558639A4

公开(公告)日：1994-02-16

申请号：EP92901130

申请日：1991-11-22

申请人： COULTER CORPORATION

发明人： HAJEK, CONSTANCE, MARY ,  RUSSELL, THOMAS

IPC分类号： G01N1/28 ,  G01N1/30 ,  G01N1/31 ,  G01N15/06 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/574

CPC分类号： G01N33/574 ,  G01N1/312 ,  G01N15/0625 ,  G01N15/147 ,  G01N15/1475 ,  G01N2015/1472

摘要： An optical screening method and apparatus (10, 28, 46) for identifying both the morphology and selected characteristics or properties expressed by cells, such as cancer cells. The cells are combined with one or more different sets of microspheres (64, 72, 76, 80, 84, 88, 92, 96, 104, 110, 116, 122, 124), each set having a reactant bond thereto which will bind to a specific molecule which can exist on one or more types of cancer cells. The cells and microspheres are formed as a smear on a slide, stained with a histological type stain and optically viewed to identify the type of cells to which the different sets of microspheres do or do not bind. The different sets of microspheres are optically differentiated by having different optical characteristics, such as size, shape, color or combinations thereof. A fast cancer cell screening method or panel is provided by adding different sets of microspheres having different reactants bound thereto to different sample portions and optically screening the different sample smears. The microspheres also can be removed from a sample portion with any cells bound thereto and compared to another non-modified sample portion to ascertain if the cancer cells of interest have been removed from the sample portion.

摘要翻译： 一种光学筛选方法和装置（10,28,46），用于鉴定由细胞（如癌细胞）表达的形态和所选特征或性质。 将细胞与一组或多组不同的微球体（64,72,76,80,84,88,92,96,104,110,116,122,124）组合，每组微球体具有与其结合的反应物键 涉及可存在于一种或多种类型的癌细胞上的特定分子。 细胞和微球体在载玻片上形成涂片，用组织学类型染色剂染色并光学观察以鉴定不同组微球体所结合或不结合的细胞的类型。 不同组的微球体通过具有不同的光学特性（例如尺寸，形状，颜色或其组合）而被光学区分。 快速癌细胞筛选方法或组通过将不同组的具有结合于其上的不同反应物的不同组的微球体添加到不同样品部分并光学筛选不同样品涂片。 微球也可以从其上结合有任何细胞的样本部分移除并与另一未修改的样本部分比较以确定感兴趣的癌细胞是否已从样本部分移除。