公开(公告)号：EP2235045B1

公开(公告)日：2012-10-03

申请号：EP08870024.0

申请日：2008-12-08

申请人： CSIR

发明人： BERGER, Eldie ,  DU PLESSIS, Erika, Margarete ,  LOUW, Maureen, Elizabeth ,  CRAMPTON, Michael, Craig ,  GERBER, Isak, Bartholomeus

IPC分类号： C07K14/31 ,  C02F1/28 ,  C02F3/34 ,  C07K19/00

CPC分类号： C07K14/32 ,  C07K2319/00

公开(公告)号：EP2235045A1

公开(公告)日：2010-10-06

申请号：EP08870024.0

申请日：2008-12-08

申请人： CSIR

发明人： BERGER, Eldie ,  DU PLESSIS, Erika, Margarete ,  LOUW, Maureen, Elizabeth ,  CRAMPTON, Michael, Craig ,  GERBER, Isak, Bartholomeus

IPC分类号： C07K14/31 ,  C02F1/28 ,  C02F3/34 ,  C07K19/00

CPC分类号： C07K14/32 ,  C07K2319/00

摘要： A method of producing a flagellin-based chimeric protein includes culturing a B. halodurans BhFD05 (Δhag, ΔfIiD, ΔwprA, ΔaIp, Δapr, Δvpr, Δasp) strain deposited under Accession Number 41533 at the NCIMB. The strain is caused to express and secrete high levels of a flagellin-based chimeric protein into an extracellular growth medium. The flagellin-based chimeric protein comprises a heterologous peptide (i) inserted in-frame into a flagellin variable region which is flanked on its N-terminal side by an N-terminal fragment of a flagellin polypeptide and, optionally, flanked on its C-terminal side by a C- terminal fragment of a flagellin polypeptide, or (ii) fused to the C-terminal of a flagellin polypeptide.