公开(公告)号：EP0717108A1

公开(公告)日：1996-06-19

申请号：EP95917990.4

申请日：1995-05-10

申请人： CYANAMID IBERICA, SA

发明人： PLANA DURAN, Juan ,  CASAL ALVAREZ, José Ignacio ,  CLIMENT SANCHEZ, Isabel

IPC分类号： C12N15/40 ,  C07K14/08 ,  C12N7/01 ,  A61K39/12 ,  G01N33/569

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/14143 ,  C12N2770/10022 ,  Y10S424/815

摘要： The present invention discloses the production of recombinant proteins of the virus causing the porcine respiratory and reproductive syndrome (PRRS), corresponding to the ORFs 2-7 of the PRRSV isolated in Spain, (PRRS-Olot), in a system of expression of recombinant baculoviruses multiplided in a cell culture of a permissive host. Said recombinant proteins are appropriate to formulate vaccines capable of efficiently protecting pigs against PRRS as well as to prepare diagnotic kits appropriate to detect both the presence of antibodies which recognize PRRSV and the presence of PRRSV in a porcine biological sample. This invention applies to veterinary.

摘要翻译： 本发明公开了在重组表达系统中产生对应于在西班牙分离的PRRSV的ORFs 2-7（PRRS-Olot）的引起猪​​呼吸和生殖综合征（PRRS）的病毒的重组蛋白质的重组蛋白质 杆状病毒在允许宿主的细胞培养物中繁殖。 所述重组蛋白质适于配制能够有效保护猪免于PRRS的疫苗，以及制备适合于检测识别PRRSV的抗体的存在以及猪生物样品中PRRSV的存在的诊断试剂盒。 本发明适用于兽医。