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1.发明授权ÉLECTROPHORÈSE CAPILLAIRE BI - OU MULTIDIMENSIONNELLE AU SEIN D'UN UNIQUE CAPILLAIRE 有权二或多维毛细管电泳一EINZELKAPILLAR
公开(公告)号：EP2080020B1
公开(公告)日：2012-03-28
申请号：EP07858445.5
申请日：2007-10-15
IPC分类号： G01N27/447
CPC分类号： G01N27/44773
摘要： The present invention relates to a method for separating the components of a mixture M by electrophoresis in a single capillary, wherein: (A) the compounds of the mixture M are introduced and migrate inside the capillary according to the capillary electrophoresis technique; (B) a fraction F of the compounds separated in step (A) is isolated in the capillary and said isolated fraction F is forced to migrate towards the vicinity of one end of the capillary while maintaining this fraction inside the capillary; (C) upon the application of a pressure difference between the ends of the capillary, a second separation medium MS2 different from the first separation medium MS1 of step (A) is introduced into the capillary through the end of the capillary in the vicinity of which the fraction F is located, whereby the fraction F migrates to the other end of the capillary while filling the capillary with the separation medium MS2; and (D) an capillary electrophoresis is carried out in the capillary thus filled with the separation medium MS2, using the medium MS2 as the separation medium.
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2.发明公开ÉLECTROPHORÈSE CAPILLAIRE BI - OU MULTIDIMENSIONNELLE AU SEIN D'UN UNIQUE CAPILLAIRE 有权二或多维毛细管电泳一EINZELKAPILLAR
公开(公告)号：EP2080020A1
公开(公告)日：2009-07-22
申请号：EP07858445.5
申请日：2007-10-15
IPC分类号： G01N27/447
CPC分类号： G01N27/44773
摘要： The present invention relates to a method for separating the components of a mixture M by electrophoresis in a single capillary, wherein: (A) the compounds of the mixture M are introduced and migrate inside the capillary according to the capillary electrophoresis technique; (B) a fraction F of the compounds separated in step (A) is isolated in the capillary and said isolated fraction F is forced to migrate towards the vicinity of one end of the capillary while maintaining this fraction inside the capillary; (C) upon the application of a pressure difference between the ends of the capillary, a second separation medium MS2 different from the first separation medium MS1 of step (A) is introduced into the capillary through the end of the capillary in the vicinity of which the fraction F is located, whereby the fraction F migrates to the other end of the capillary while filling the capillary with the separation medium MS2; and (D) an capillary electrophoresis is carried out in the capillary thus filled with the separation medium MS2, using the medium MS2 as the separation medium.
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