公开(公告)号：EP3839484A1

公开(公告)日：2021-06-23

申请号：EP19306658.6

申请日：2019-12-17

申请人： Centre National de la Recherche Scientifique ,  Ecole Normale Supérieure de Paris ,  SORBONNE UNIVERSITE

发明人： CHOUKET, Raja ,  CROQUETTE Vincent ,  ESPAGNE, Agathe ,  JULLIEN, Ludovic ,  LEMARCHAND Annie ,  LE SAUX Thomas ,  PELISSIER-TANON, Agnés ,  ZHANG, Ruikang

IPC分类号： G01N21/64

摘要： A method for detecting a reversibly photoswitchable chemical species in a sample, comprising the steps of:
a) illuminating the sample (S) with light suitable to be absorbed by the chemical species triggering a reaction affecting an optical property of the chemical species, said first light being periodically-modulated at a fundamental modulation frequency;
b) measuring the evolution of the optical property;
c) extracting at least one of an in-phase component at a frequency which is an even multiple of the fundamental modulation frequency; and a quadrature component at a frequency which is an odd multiple of the fundamental modulation frequency of a signal representing said evolution; and
d) using the extracted component or components for detecting the chemical species.
An apparatus for carrying out the method.