公开(公告)号：EP2699353A1

公开(公告)日：2014-02-26

申请号：EP12720642.3

申请日：2012-04-20

申请人： Centre National de la Recherche Scientifique (CNRS) ,  Ecole Normale Supérieure ,  Université Pierre et Marie Curie (Paris 6) ,  Fonds de l'ESPCI - Georges Charpak ,  Université Bordeaux Segalen

发明人： DAHAN, Maxime ,  MOREL, Mathieu ,  GALAS, Jean-Christophe ,  BARTOLO, Denis ,  STUDER, Vincent

IPC分类号： B01L3/00 ,  C12M3/06 ,  G01N33/50

CPC分类号： G01N33/5008 ,  B01J2219/00639 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/087 ,  B01L2300/163 ,  C12M23/16

摘要： The invention relates to a microfluidic system for controlling a card for the concentration of molecules capable of stimulating a target, for example formed by an assembly of living cells, characterized in that the system comprises a microfluidic device (1) comprising:
n c ≥ 1 microfluidic channel(s) (4, 40), the or each channel being provided with at least one inlet orifice for at least one fluid and with at least one outlet orifice for this fluid;
n 0 ≥ 2 openings (47, 470) formed in the microfluidic channel or distributed in the various microfluidic channels, said openings being arranged in one and the same plane so that they form a network having at least one dimension in this plane, the numbers
n c of microfluidic channel(s) and
n 0 of openings being linked by the relationship (I) with 1 ≤ i ≤
n c and
n 0/ci the number of openings for the channel
c i ; at least one microporous membrane (5) covering the network of openings, the target being intended to be positioned on the side of the membrane which is opposite the microfluidic channel(s); one or more fluid feed means for feeding the or each microfluidic channel with fluid, at least one of these fluids comprising molecules for stimulating the target.

公开(公告)号：EP2680971A1

公开(公告)日：2014-01-08

申请号：EP12708969.6

申请日：2012-03-02

申请人： Centre National de la Recherche Scientifique ,  Ecole Normale Supérieure ,  Université Pierre et Marie Curie (Paris 6)

发明人： DAHAN, Maxime ,  MOREL, Mathieu ,  GALAS, Jean-Christophe ,  STUDER, Vincent ,  BARTOLO, Denis

IPC分类号： B01L3/00 ,  C12M3/06 ,  G01N33/50

CPC分类号： G01N33/5008 ,  B01J2219/00639 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/087 ,  B01L2300/163 ,  C12M23/16

摘要： The invention relates to a microfluidic system for controlling a concentration profile of molecules capable of stimulating a target, for example formed by an assembly of living cells, this system comprising: - a microfluidic device (1) comprising at least one microfluidic channel (4) equipped with at least one inlet orifice (21) and with at least one outlet orifice (22) for at least one fluid; - at least one means for supplying the microfluidic channel (4) with at least one fluid comprising molecules capable of stimulating the target; - at least one chamber (8) or another microfluidic channel comprising a base (6) intended to receive the target; and - at least one microporous membrane (5) separating the chamber (8) or the other microfluidic channel from the microfluidic channel (4), said microporous membrane (5) being positioned away from the base (6) so that when the supply means provides the microfluidic channel (4) with said at least one fluid flowing in laminar flow in contact with the microporous membrane (5), the molecules capable of stimulating the target then diffuse, after having passed through the microporous membrane (5) through the chamber (8) or said other microfluidic channel in order to finally form a stable concentration profile in this chamber (8) or this other microfluidic channel.