公开(公告)号：EP4101934A1

公开(公告)日：2022-12-14

申请号：EP21305779.7

申请日：2021-06-08

申请人： Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) ,  Université de La Réunion

发明人： ROBENE, Isabelle ,  JOUEN, Emmanuel ,  HASCOAT, Jérémy ,  CETRE-SOSSAH, Catherine ,  REYNAUD, Bernard ,  LEBON, Véronique ,  FENELON, Babbitha

IPC分类号： C12Q1/6844 ,  C12Q1/70

摘要： The invention relates to a method of detecting the presence of a SARS coronavirus nucleic acid, comprising:
- contacting the biological sample with a sample buffer comprising a set of loop-mediated isothermal amplification primers specific for the SARS nucleic acid under conditions sufficient for a loop-mediated isothermal amplification, i.e. LAMP, of the SARS nucleic acid, wherein the set of LAMP primers comprises six primers of SEQ ID NOs: 1-7, thereby producing a SARS amplification sample;
- carrying out the LAMP from the SARS amplification sample, thereby producing a SARS amplification product; and
- detecting the SARS amplification product, thereby detecting the presence of the SARS nucleic acid in the sample.