公开(公告)号：EP4407317A2

公开(公告)日：2024-07-31

申请号：EP24181937.4

申请日：2018-03-28

申请人： Charles River Laboratories, Inc.

发明人： SCHOLLE, Michael D. ,  SHERRILL, John

IPC分类号： G01N33/68

CPC分类号： C12Q1/6816 ,  C40B30/04 ,  G01N33/6851

摘要： Articles and methods involving detection of binding between two or more species using MALDI are generally provided. In some embodiments, a method comprises exposing a first species to a composition comprising a second species, capturing the first species on a self-assembled monolayer, and performing MALDI on the self-assembled monolayer to generate a signal from the second species and a signal from a standard species present in the self-assembled monolayer. A ratio (first ratio) of the signal from the second species to the signal from the standard species may be compared to a cutoff value in order to determine if binding has occurred. This method may allow determination of a relative affinity of the second species for the first species.

公开(公告)号：EP4407317A3

公开(公告)日：2024-10-23

申请号：EP24181937.4

申请日：2018-03-28

申请人： Charles River Laboratories, Inc.

发明人： SCHOLLE, Michael D. ,  SHERRILL, John

IPC分类号： G01N33/68 ,  C12Q1/6816 ,  C40B30/04

摘要： Articles and methods involving detection of binding between two or more species using MALDI are generally provided. In some embodiments, a method comprises exposing a first species to a composition comprising a second species, capturing the first species on a self-assembled monolayer, and performing MALDI on the self-assembled monolayer to generate a signal from the second species and a signal from a standard species present in the self-assembled monolayer. A ratio (first ratio) of the signal from the second species to the signal from the standard species may be compared to a cutoff value in order to determine if binding has occurred. This method may allow determination of a relative affinity of the second species for the first species.