公开(公告)号：EP1766036A1

公开(公告)日：2007-03-28

申请号：EP05762102.1

申请日：2005-07-01

申请人： Cobra Biologics Limited

发明人： CRANENBURGH, Rocky Marc Cobra Biologics Limited ,  BLOOR, Alexandra Elizabeth Cobra Biologics Limited

IPC分类号： C12N15/90

CPC分类号： C12N15/86 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2800/30

摘要： The invention relates to a process for the removal of selectable marker gene sequences, in particular antibiotic gene sequences, from nucleic acid molecules, wherein the process makes use of dif-like site-specific recombinase recognition sites. The invention further relates to the application of this process in the unlabelled integration and deletion of chromosomal genes and in controlling gene expression.