专利检索 ap:("Codon Devices, Inc.") AND inv:"AFEYAN, Noubar" 第 1 页

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发明公开
METHODS FOR ASSEMBLY OF HIGH FIDELITY SYNTHETIC POLYNUCLEOTIDES 审中-公开
标题翻译：方法进行组装高保真合成的多核苷酸的

公开(公告)号：EP1812598A1

公开(公告)日：2007-08-01

申请号：EP05815025.1

申请日：2005-10-18

申请人： Codon Devices, Inc.

发明人： AFEYAN, Noubar , CHURCH, George , JACOBSON, Joseph , BAYNES, Brian, M. , NESMITH, Kenneth, Gabriel , CHAPMAN, Brad, Alan , STRACK-LOGUE, Bettina

IPC分类号： C12Q1/68

CPC分类号： C12N15/66 , C12N15/10 , C12Q1/6844 , C12Q2531/113 , C12Q2521/514 , C12Q2533/107 , C12Q2521/307 , C12Q2533/101

摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of nucleic acid polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve preamplification of one or more oligonucleotides using 'universal' primers, reduction of the error rate in oligonucleotide and/or nucleic acid products, and sequence optimization and oligonucleotides design. Also provided are low-purity arrays of nucleic acids and methods for assembling nucleic acids using oligonucleotides obtained from low-purity arrays.