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公开(公告)号：EP2267138B1
公开(公告)日：2016-06-08
申请号：EP10183696.3
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
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公开(公告)号：EP2267139A3
公开(公告)日：2011-05-25
申请号：EP10183703.7
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with the sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprise simultaneously both sense and antisense nucleotide sequence.
摘要翻译： 提供了通过引入编码针对靶核酸的有义和反义RNA分子的嵌合基因来减少真核细胞，特别是植物细胞中目标核酸的表型表达的方法和手段，所述嵌合基因能够形成双链 通过区域与有义和反义核苷酸序列之间的碱基配对或通过引入RNA分子本身来实现RNA区域。 优选地，RNA分子同时包含有义和反义核苷酸序列。
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公开(公告)号：EP2267138A3
公开(公告)日：2011-05-25
申请号：EP10183696.3
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with the sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprise simultaneously both sense and antisense nucleotide sequence.
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公开(公告)号：EP2267139A2
公开(公告)日：2010-12-29
申请号：EP10183703.7
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with the sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprise simultaneously both sense and antisense nucleotide sequence.
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5.发明公开Methods and means for obtaining modified phenotypes 有权Verfahren und Mittel zum Erhalt von modifiziertenPhänotypen
公开(公告)号：EP2267138A2
公开(公告)日：2010-12-29
申请号：EP10183696.3
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with the sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprise simultaneously both sense and antisense nucleotide sequence.
摘要翻译： 提供了通过引入编码针对靶核酸的正义和反义RNA分子的嵌合基因，其能够形成双链的方法和装置，用于在真核细胞，特别是植物细胞中减少感兴趣核酸的表型表达 RNA区域通过有义和反义核苷酸序列的区域之间的碱基配对或通过引入RNA分子本身。 优选地，RNA分子同时包含有义和反义核苷酸序列。
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