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    Microplates with UV permeable bottom wells and methods of making same
    1.
    发明公开
    Microplates with UV permeable bottom wells and methods of making same 无效
    Mikroplatten mit UVdurchsichtigenBecherbödenund Verfahren zu deren Herstellung

    公开(公告)号：EP0797088A1

    公开(公告)日：1997-09-24

    申请号：EP97301686.8

    申请日：1997-03-13

    申请人： Corning Costar Corporation

    发明人： Mathus, Gregory ,  Szlosek R.R., Paul M. ,  Lacey, William J.

    IPC分类号： G01N21/25 ,  B01L3/00 ,  G01N21/03

    CPC分类号： B01L3/5085 ,  B01L2300/0829 ,  B01L2300/12 ,  B29C45/1418 ,  B29C45/14344 ,  B29C45/14778 ,  G01N21/03 ,  G01N21/25 ,  G01N21/33

    摘要： Microplates and methods for manufacturing microplates. The microplate is designed to allow UV radiation to pass through the bottom wells of the microplate so that the microplate can be used for assaying samples by use of UV absorbance. In one embodiment, the microplate comprises at least first and second wells, each well having a UV permeable bottom. In another embodiment, the microplate comprises a frame having an upper portion and a lower portion contiguous with the upper portion and a sheet disposed between the upper portion and the lower portion and defining the bottom of at least one well of the microplate. One embodiment of the method includes steps of inserting a sheet of UV permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten plastic material into the mold cavity, and cooling the plastic material to form the microplate with the plastic material forming the sidewalls of each of the first and second wells and the sheet of UV permeable material forming the bottom of each of the first and second wells. Another embodiment of the method includes providing an upper plate defining sidewalls of at least one well, adhering an intermediate layer to the upper plate and adhering a sheet of UV permeable material to the intermediate layer. A further embodiment of the method includes inserting a sheet of material having at least one hole into a mold cavity, injecting a molten plastic material into the mold cavity and cooling the plastic material to form a microplate.

    摘要翻译： 微孔板和微孔板制造方法。 微孔板被设计成允许UV辐射通过微板的底部孔，使得微孔板可以用于通过使用UV吸光度来测定样品。 在一个实施方案中，微孔板包括至少第一和第二孔，每个孔具有可UV透过的底部。 在另一个实施例中，微板包括具有上部和与上部相邻的下部的框架，以及设置在上部和下部之间并限定微板的至少一个孔的底部的框架。 该方法的一个实施方案包括以下步骤：将一片UV可渗透材料插入到模腔中，该模腔包括成形为形成多个孔的侧壁的部分，将熔融的塑料材料注入模腔中，并且冷却塑料材料以形成 微孔板，其中塑料材料形成第一和第二孔中的每一个的侧壁，以及形成第一和第二孔中的每一个的底部的UV可渗透材料片。 该方法的另一实施例包括提供限定至少一个阱的侧壁的上板，将中间层粘附到上板并将一片UV可渗透材料粘合到中间层。 该方法的另一实施例包括将具有至少一个孔的材料片插入模腔中，将熔融的塑料材料注入模腔并冷却塑料材料以形成微板。